Ovariectomy increases the level of estrogen receptor mRNA and estrogen receptor binding sites in female rat adipose tissue
| Autor: | Hiromasa Ikegami, Yoshinobu Nishikawa, Masahiro Sakata, Kenji Hirota, Takahiro Mizutani, Hirohisa Kurachi, Kenichiro Morishige, A. Miyake, Osamu Tanizawa |
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| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Endocrinology Diabetes and Metabolism Ovariectomy Adipose tissue Estrogen receptor Biology Endocrinology Cytosol Internal medicine medicine Animals Northern blot RNA Messenger Binding site Rats Wistar In Situ Hybridization Messenger RNA Estradiol Estrogen receptor binding Body Weight Biological activity Blotting Northern Rats Adipose Tissue Receptors Estrogen Estrogen Female hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of endocrinological investigation. 16(8) |
| ISSN: | 0391-4097 |
| Popis: | The roles of estrogen in the changes in estrogen receptor (ER) mRNA and ER binding sites in rat adipose tissue were studied in female rats. To elucidate the mechanism(s) behind ovariectomy (OVX)-induced obesity, the levels of ER mRNA and ER binding sites in adipose tissue were analyzed three weeks after OVX using Northern blot analysis of ER mRNA and the [3H]E2 binding assay, respectively. OVX induced an increase in body weight, and replacement of estradiol (E2) prevented that increase. Significant increases in the amounts of ER mRNA and in [3H]E2-specific binding were observed after OVX, and E2 replacement reduced both of those increases. These results suggest that E2 may regulate rat obesity directly through ER in adipose tissues. |
| Databáze: | OpenAIRE |
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