2-Aminofluorene-DNA adducts in mouse urinary bladder: effect of age, sex and acetylator phenotype
| Autor: | Wendell W. Weber, Gerald N. Levy |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Mice Inbred A DNA damage Urinary Bladder Congenic Adduct Lesion DNA Adducts Mice chemistry.chemical_compound Sex Factors Internal medicine medicine Animals Chromatography High Pressure Liquid Carcinogen Genetics Fluorenes Urinary bladder Chemistry Age Factors Acetylation DNA General Medicine Mice Inbred C57BL Phenotype Endocrinology medicine.anatomical_structure Toxicity Carcinogens Female medicine.symptom Injections Intraperitoneal DNA Damage |
| Zdroj: | Carcinogenesis. 13:159-164 |
| ISSN: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/13.2.159 |
| Popis: | Formation of urinary bladder DNA-2-aminofluorene adducts in inbred and acetylator congenic mice was measured 3 h after a 60 mg/kg dose of the arylamine carcinogen. The sensitivity of 32P-postlabeling with HPLC analysis permitted quantitation of adducts in individual mouse bladders. Acetylator phenotype was a significant determinant of DNA damage in female mice as slow acetylators had higher levels of bladder DNA adducts than rapids. This correlation is the reverse of that seen with hepatic DNA. Age was also a significant determinant of DNA damage as older mice (20-23 weeks) formed more bladder DNA adducts than young (7 week) mice. The age-related increase in bladder adduct formation was seen in both sexes of all mouse lines. Male B6 mice exposed to 2-aminofluorene at 20-23 weeks of age showed a 26-fold higher level of bladder DNA adducts than males exposed at 7 weeks. In addition to the large increase in total adduct level, the older male B6 mice produced significant amounts of an unidentified, early-eluting adduct peak that had chromatographic properties similar to an aminofluorene-DNA adduct produced through peroxidative activation. These results indicate that age, sex and acetylator phenotype are all important determinants of aromatic amine-bladder DNA adduct formation in mice. |
| Databáze: | OpenAIRE |
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