Current approaches in identification and isolation of human renal cell carcinoma cancer stem cells
| Autor: | Mohammed Imran Khan, Ewa Bartnik, Igor Helbrecht, Cezary Szczylik, Anna M. Czarnecka, Fei Lian |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
medicine.diagnostic_test
Somatic cell Primary Cell Culture Medicine (miscellaneous) Cell Biology Review Cell sorting Biology Flow Cytometry Biochemistry Genetics and Molecular Biology (miscellaneous) CXCR4 Flow cytometry Side population Cell culture Cancer stem cell Immunology Cancer research medicine Biomarkers Tumor Neoplastic Stem Cells Molecular Medicine Humans Stem cell Carcinoma Renal Cell |
| Zdroj: | Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| DOI: | 10.1186/s13287-015-0177-z |
| Popis: | In recent years, cancer stem cells (CSCs)/tumor initiating cells (TICs) have been identified inside different tumors. However, currently used anti-cancer therapies are mostly directed against somatic tumor cells without targeting CSCs/TICs. CSCs/TICs also gain resistance to chemotherapies/radiotherapies. For the development of efficient treatment strategies, choosing the best method for isolation and characterization of CSCs/TICs is still debated among the scientific community. In this review, we summarize recent data concerning isolation techniques for CSCs using magnetic cell sorting and flow cytometry. The review focuses on the strategies for sample preparation during flow cytometric analysis, elaborating biomarkers such as CXCR4, CD105, and CD133. In addition, functional properties characteristic of CSCs/TICs using side population selection through Hoechst 33342 dye, aldehyde dehydrogenase 1, dye-cycle violet, and rhodamine 123 are also discussed. We also include a special focus on enriching CSCs/TICs using three-dimensional cell culture models such as agarose–agarose microbeads and sphere formation. |
| Databáze: | OpenAIRE |
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