Additional file 2 of Cell-free culture supernatants of Lactobacillus spp. and Pediococcus spp. inhibit growth of pathogenic Escherichia coli isolated from pigs in Thailand

Autor: Kaewchomphunuch, Thotsapol, Charoenpichitnunt, Thunyathorn, Thongbaiyai, Varissara, Ngamwongsatit, Natharin, Kaeoket, Kampon
Rok vydání: 2022
DOI: 10.6084/m9.figshare.19092348.v1
Popis: Additional file 2: Figure S1. The Cell-free culture supernatant preparation procedures, the cultivation of LAB in MRSC broth were inoculated overnight (1-A). Centrifugation process of LAB broth (1-B), Contents in sample tube separated into two parts supernatant (liquid part) and precipitation after centrifugation (1-C). Supernatant was drained out into sterile syringe (1-D). The filtration process was performed with sterile PES membrane (1-E). The CFCS was completely prepared and ready to use (1-F). Figure S2. The demonstration of agar well diffusion assay. Figure S3. Agar well diffusion assay, the presence of ZOI from CFCS against pathogenic E. coli V13-2LF2 at 8 h incubation (3-A). Meanwhile, the presence of ZOI from CFCS was significantly decreased at 16 h incubation (3-B). Figure S4. The cross-streaking assay, an illustration of 3 different patterns: pattern 1 (4-A), pattern 2 (4-B), and pattern 3 (4-C) in experiment and demonstration of 6 streaked lines of LAB. Close contact points are marked as a black arrow.
Databáze: OpenAIRE