The Effect of Including the C2 Insert of Nonmuscle Myosin II-C on Neuritogenesis
| Autor: | Provas Das, Shekhar Saha, Mahua Rani Das, Arunima Biswas, Siddhartha S. Jana, Sumit K. Dey |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Gene isoform
Neurite Cellular differentiation macromolecular substances Myosins Biology Transfection Models Biological Biochemistry Cell Line Mice Myosin Neurites Animals Protein Isoforms Pseudopodia RNA Small Interfering Molecular Biology Myosin Type II Neurons Myosin Heavy Chains Colocalization Cell Differentiation Cell Biology Cell biology Alternative Splicing Microscopy Fluorescence Cell culture |
| Zdroj: | Journal of Biological Chemistry. 288:7815-7828 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m112.417196 |
| Popis: | The functional role of the C2 insert of nonmuscle myosin II-C (NM II-C) is poorly understood. Here, we report for the first time that the expression of the C2 insert-containing isoform, NM II-C1C2, is inducible in Neuro-2a cells during differentiation both at mRNA and protein levels. Immunoblot and RT-PCR analysis reveal that expression of NM II-C1C2 peaks between days 3 and 6 of differentiation. Localization of NM II-C1C2 in Neuro-2a cells suggests that the C2 insert-containing isoform is localized in the cytosol and along the neurites, specifically at the adherence point to substratum. Inhibition of endogenous NM II-C1C2 using siRNA decreases the neurite length by 43% compared with control cells treated with nonspecific siRNA. Time lapse image analysis reveals that neurites of C2-siRNA-treated cells have a net negative change in neurite length per minute, leading to a reduction of overall neurite length. During neuritogenesis, NM II-C1C2 can interact and colocalize with β1-integrin in neurites. Altogether, these studies indicate that NM II-C1C2 may be involved in stabilizing neurites by maintaining their structure at adhesion sites. |
| Databáze: | OpenAIRE |
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