Glycine preconditioning to ameliorate pulmonary ischemia reperfusion injury in rats

Autor: Stefanie Sommer, Sebastian-Patrick Sommer, Bhanu Sinha, Rainer Leyh
Přispěvatelé: Microbes in Health and Disease (MHD), Critical care, Anesthesiology, Peri-operative and Emergency medicine (CAPE)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
Time Factors
Wistar
Apoptosis
Pharmacology
Mitochondrion
New Ideas
Inner mitochondrial membrane
Lung
Membrane Potential
Mitochondrial
biology
integumentary system
Cytochrome c
Respiratory chain complex
Cytochromes c
Pulmonary edema
Glutathione
Mitochondrial
Mitochondria
Matrix Metalloproteinase 9
Neutrophil Infiltration
Reperfusion Injury
embryonic structures
Cardiology and Cardiovascular Medicine
Oxidation-Reduction
Pulmonary and Respiratory Medicine
medicine.medical_specialty
animal structures
Ischemia
Glycine
Pulmonary Edema
Protective Agents
Membrane Potential
medicine
Animals
Rats
Wistar
business.industry
Animal
medicine.disease
Surgery
Rats
Enzyme Activation
Disease Models
Animal
Electron Transport Chain Complex Proteins
Cytoprotection
Disease Models
biology.protein
business
Reperfusion injury
Zdroj: Interactive Cardiovascular and Thoracic Surgery, 14(5), 521-525. Oxford University Press
ISSN: 1569-9293
Popis: This study examines the impact of glycine (Gly) preconditioning on ischemia reperfusion (IR)-induced pulmonary mitochondrial injury to research the previously, in pig lungs, demonstrated Gly-dependent amelioration of pulmonary IR injury. IR injury was induced in rat lungs by 30 min pulmonary hilum clamping followed by 60 min reperfusion time. Rats were subjected to controls, shams and two study groups (IR30/60, Gly-IR30/60) receiving 37.5 mg Gly i.v. or not before IR induction. The wet/dry-weight ratio, mitochondria viability (MV), membrane integrity (MI), respiratory chain complex (RCC) activities, mitochondrial membrane potential (ΔΨm) and cytochrome C (Cyt C) content were analysed. In IR30/60, RCC and MV were impaired; Cyt C loss and MI combined with matrix metalloproteinase-9 (MMP-9) activation and ΔΨm alteration were observed when compared with controls. In Gly-IR30/60, complex II function and mitochondrial viability were protected during IR, and MMP-9 activation combined with tissue-water content accumulation and ΔΨm alteration were ameliorated. Cyt C loss, mitochondrial membranes damage, tissue GSH oxidation or neutrophil sequestration was not extenuated in Gly-IR30/60. Gly ameliorates IR-associated mitochondrial dysfunction and decay of viability and normalizes ΔΨm but does not protect from Cyt C liberation and mitochondrial membrane damage. Our data suggest that the previously described effect of Gly preconditioning results at least partially from mitochondrial protection. A dose-finding study is necessary to improve results of Gly preconditioning.
Databáze: OpenAIRE
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