Influence of preadipocyte-conditioned medium on the proliferation and invasive potential of breast cancer cell lines in vitro
| Autor: | Jan Scheich, Tobias Hirsch, Frank Jacobsen, Clemens B. Tempfer, Anna Jablonka, Marcus Lehnhardt, Günther A. Rezniczek, Mazen Hagouan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Eotaxin medicine.medical_treatment Adipose tissue Breast Neoplasms In Vitro Techniques Andrology 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Line Tumor Medicine Autologous transplantation Humans Neoplasm Invasiveness skin and connective tissue diseases Cell Proliferation business.industry Obstetrics and Gynecology Cell migration General Medicine Middle Aged medicine.disease Transplantation 030104 developmental biology Cytokine 030220 oncology & carcinogenesis MCF-7 Cells Female Stem cell business |
| Zdroj: | Archives of gynecology and obstetrics. 298(6) |
| ISSN: | 1432-0711 |
| Popis: | Autologous transplantation of adipose tissue into the breast is commonly performed in clinical practice, but its oncological safety has not been established. We conducted an in vitro study to assess the influence of factors released by adipose-derived stem cells (ASCs), from multiple source tissues and harvested using different techniques, on proliferation and invasiveness of two breast cancer cell lines. Fat specimens of 66 donors (57 female, 9 male) were collected and 44 ASC cultures were established. ASC conditioning of the medium (CM) increased the proliferation of MCF-7 cells (178.4 ± 62.8%; P |
| Databáze: | OpenAIRE |
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