Reduced skeletal-muscle perfusion and impaired ATP release during hypoxia and exercise in individuals with type 2 diabetes

Autor: Ylva Hellsten, Trine Alma Knudsen, Martin BIllmann Groen, Stefan P. Mortensen, Stine Louise Høyer Finsen, Bente Klarlund Pedersen
Rok vydání: 2019
Předmět:
Male
Adult
0301 basic medicine
medicine.medical_specialty
Microdialysis
Adenosine Triphosphate/metabolism
Endocrinology
Diabetes and Metabolism
030209 endocrinology & metabolism
Vasodilation
Type 2 diabetes
03 medical and health sciences
Adenosine Triphosphate
0302 clinical medicine
Hypoxia/metabolism
Internal medicine
Exercise/physiology
Internal Medicine
medicine
Humans
Regional Blood Flow/physiology
Exercise physiology
Hypoxia
Muscle
Skeletal
Exercise
Vasodilation/physiology
business.industry
Blood Flow Velocity/physiology
Venous Plasma
Middle Aged
Hypoxia (medical)
medicine.disease
Diabetes Mellitus
Type 2/metabolism
Microvascular disease
030104 developmental biology
Endocrinology
Diabetes Mellitus
Type 2
Regional Blood Flow
Muscle
Skeletal/blood supply
Female
Sodium nitroprusside
medicine.symptom
business
Perfusion
Blood Flow Velocity
Human
Metabolic physiology in vivo
medicine.drug
Zdroj: Groen, M B, Knudsen, T A, Finsen, S H, Pedersen, B K, Hellsten, Y & Mortensen, S P 2019, ' Reduced skeletal-muscle perfusion and impaired ATP release during hypoxia and exercise in individuals with type 2 diabetes ', Diabetologia, vol. 62, no. 3, pp. 485-493 . https://doi.org/10.1007/s00125-018-4790-0
ISSN: 1432-0428
0012-186X
DOI: 10.1007/s00125-018-4790-0
Popis: AIMS/HYPOTHESIS: Plasma ATP is a potent vasodilator and is thought to play a role in the local regulation of blood flow. Type 2 diabetes is associated with reduced tissue perfusion. We aimed to examine whether individuals with type 2 diabetes have reduced plasma ATP concentrations compared with healthy control participants (case-control design).METHODS: We measured femoral arterial and venous plasma ATP levels with the intravascular microdialysis technique during normoxia, hypoxia and one-legged knee-extensor exercise (10 W and 30 W) in nine participants with type 2 diabetes and eight control participants. In addition, we infused acetylcholine (ACh), sodium nitroprusside (SNP) and ATP into the femoral artery to assess vascular function and ATP signalling.RESULTS: Individuals with type 2 diabetes had a lower leg blood flow (LBF; 2.9 ± 0.1 l/min) compared with the control participants (3.2 ± 0.1 l/min) during exercise (p CONCLUSIONS/INTERPRETATION: These findings demonstrate that individuals with type 2 diabetes have lower plasma ATP concentrations during exercise and hypoxia compared with control individuals, and this occurs in parallel with lower blood flow. Moreover, individuals with type 2 diabetes have a reduced vasodilatory response to infused ATP. These impairments in the ATP system are both likely to contribute to the reduced tissue perfusion associated with type 2 diabetes.TRIAL REGISTRATION: ClinicalTrials.gov NCT02001766.
Databáze: OpenAIRE
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