Nicotine Treatment Induces Expression of Matrix Metalloproteinases in Human Osteoblastic Saos-2 Cells
| Autor: | Tomoko Katono, Masao Maeno, Naoto Suzuki, Hitoshi Oka, Natsuko Tanabe, Kazuhiro Yamanaka, Masafumi Motohashi, Takayuki Kawato |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Macrophage colony-stimulating factor
Nicotine Biophysics Matrix metalloproteinase Biochemistry Dinoprostone Plasminogen Activators chemistry.chemical_compound Cell Line Tumor Matrix Metalloproteinase 13 Gene expression medicine Humans Prostaglandin E2 Osteoblasts Chemistry General Medicine Ganglionic Stimulants Molecular biology Matrix Metalloproteinases Cell culture Tissue Plasminogen Activator Plasminogen activator inhibitor-1 Matrix Metalloproteinase 2 Matrix Metalloproteinase 3 Matrix Metalloproteinase 1 Proto-Oncogene Proteins c-fos Plasminogen activator medicine.drug |
| Zdroj: | Acta Biochimica et Biophysica Sinica. 38:874-882 |
| ISSN: | 1672-9145 |
| DOI: | 10.1111/j.1745-7270.2006.00240.x |
| Popis: | Tobacco smoking is an important risk factor for the development of severe periodontitis. Recently, we showed that nicotine affected mineralized nodule formation, and that nicotine and lipopolysaccharide stimulated the formation of osteoclast-like cells by increasing production of macrophage colony-stimulating factor (M-CSF) and prostaglandin E2 (PGE2) by human osteoblastic Saos-2 cells. In the present study, we examined the effects of nicotine on the expression of matrix metalloproteinases (MMPs), tissue inhibitors of matrix metalloproteinases (TIMPs), the plasminogen activation system including the component of tissue-type plasminogen activator (tPA), urokinase-type PA (uPA), and PA inhibitor type 1 (PAI-1), alpha7 nicotine receptor, and c-fos. We also examined the effect of the nicotine antagonist D-tubocurarine on nicotine-induced expression of MMP-1. Gene expression was examined using real-time polymerase chain reaction (PCR) to estimate mRNA levels. In addition, expression of the MMP, TIMP, uPA, tPA, and PAI-1 proteins was determined by Western blotting analysis. Nicotine treatment caused expression of MMP-1, 2, 3, and 13, but not MMP-14, to increase significantly after 5 or 10 d of culture; MMP-14 expression did not change through day 14. Enhancement of MMP-1 expression by nicotine treatment was eliminated by simultaneous treatment with D-tubocurarine. In the presence of nicotine, expression of uPA, PAI-1, or TIMP-1, 2, 3, or 4 did not change over 14 d of culture, whereas expression of tPA increased significantly by day 7. Nicotine also increased expression of the alpha7 nicotine receptor and c-fos genes. These results suggest that nicotine stimulates bone matrix turnover by increasing production of tPA and MMP-1, 2, 3, and 13, thereby tipping the balance between bone matrix formation and resorption toward the latter process. |
| Databáze: | OpenAIRE |
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