Study of Flebogrif®—A New Tool for Mechanical Sclerotherapy—Effectiveness Assessment Based on Animal Model
| Autor: | Albert Czerski, Katarzyna Wiglusz, Maciej Dobrzyński, Agnieszka Noszczyk-Nowak, Rafal J. Wiglusz, Maria Szymonowicz, Maciej Janeczek, Marta Wujczyk, Zbigniew Rybak, Aleksandra Sender-Janeczek, Piotr Kuropka |
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| Rok vydání: | 2021 |
| Předmět: |
General Chemical Engineering
medicine.medical_treatment Lumen (anatomy) 030204 cardiovascular system & hematology Flebogrif® Article lcsh:Chemistry experimental surgery 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Animal model Hemorrhoids Occlusion Varicose veins Sclerotherapy medicine mechanochemical ablation General Materials Science Vein business.industry animal model medicine.disease endovenous procedure medicine.anatomical_structure Lymphatic system lcsh:QD1-999 medicine.symptom business Nuclear medicine |
| Zdroj: | Nanomaterials Volume 11 Issue 2 Nanomaterials, Vol 11, Iss 544, p 544 (2021) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano11020544 |
| Popis: | Sclerotherapy is the chemical occlusion of vessels using an intravenous injection of a liquid or foamed sclerosing agent that is used in the therapy of blood and lymphatic vessels malformations in the young, and for spider veins, smaller varicose veins, hemorrhoids and hydroceles in adults. This study aimed to assess the effectiveness of mechanosclerotherapy of venous veins with a new device—Flebogrif®—based on an animal model. The experiment was performed on nine Polish Merino sheep weighing 40–50 kilograms. The animals were anesthetized intravenously. The material was divided into three groups: two experimental (1 and 2) and control (3) group. The first experimental group was treated with the use of Flebogrif® and a sclerosant simultaneously, while only Flebogrif® was used in the second experimental group. Flebogrif® was applied into the lateral saphenous vein of both pelvic limbs. The vessel wall thickness was estimated at four points of the histological image in mm (V1, V2, V3, V4). For one month, the animals were euthanized, and the occlusion rate of the treated veins and changes in the vein wall were determined. Histological slides were analyzed under a light microscope and histometry of the vein wall was performed. The Shapiro–Wilk test and the quantity of the investigated parameter groups allowed for using a non-parametric method at four points to compare thickness measurements (the Mann–Whitney test), with p < 0.05. The Mann–Whitney test indicated statistically significant differences between both experimental groups. The results obtained from morphometrical and histological analysis showed better results in the first experimental group than those of the second experimental group. Finally, statistical analysis revealed significant differences between the both the experimental group and control group in morphological analysis. The achieved results allowed us to conclude that the simultaneous use of Flebogrif® and a sclerosant yielded better results of vein lumen reduction than the use of Flebogrif® alone. |
| Databáze: | OpenAIRE |
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