Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells
| Autor: | Sara De Martin, Daniela Gabbia, Franco Biasioli, Sara Bogialli, Daniela Rainer, Ronald Gstir, Luca Cappellin, Andrea Boschetti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis Cytotoxicity 010501 environmental sciences Pharmacology Lung injury Toxicology 01 natural sciences Nicotine 03 medical and health sciences 0302 clinical medicine RA1190-1270 Refill liquids medicine EVALI e-vaping acute lung injury HUVEC human umbilical vein endothelial cells Settore VET/07 - FARMACOLOGIA E TOSSICOLOGIA VETERINARIA 0105 earth and related environmental sciences ComputingMethodologies_COMPUTERGRAPHICS e-Cig electronic cigarette LDH - Lactate dehydrogenase LDH lactate dehydrogenase Chemistry EVALI Regular Article ENDS electronic nicotine delivery systems Ldh release Electronic cigarettes Toxicity Toxicology. Poisons 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Toxicology Reports Toxicology Reports, Vol 8, Iss, Pp 456-462 (2021) |
| ISSN: | 2214-7500 |
| Popis: | Graphical abstract Highlights • The vapors obtained from different refill liquids for e-cigarettes exert different extent of cytotoxicity. • The condensates obtained from traditional cigarettes are always highly cytotoxic in the same experimental conditions. • Accurate evaluations for refill liquids will help to correlate toxicity to chemical composition. The electronic cigarettes mimic combustible cigarettes through a heating technology that vaporizes a refill liquid consisting of solvents, flavors, and nicotine. E-cigarettes are sometimes still used as a support for smoking cessation, even if in 2019 an acute lung injury outbreak occurred in the USA, affecting mainly adolescents and young adults, and was correlated to eCigs. Therefore, due to the lack of a definite knowledge about the mechanism(s) of refill liquid toxicity and considering that previous investigations gave controversial results, the aim of the present study was the cytotoxicity assessment of different refill liquids on human endothelial cells, evaluated by means of two different in vitro approaches, i.e. the resazurin and the LDH release assays. Our results clearly demonstrated that different refill liquids (6 samples) display different levels of cytotoxicity in our cellular model, although their cytotoxicity was always lower than that observed for the condensate obtained from traditional cigarettes (3 samples). These results suggest that accurate evaluations should be provided for refill liquids, in particular to correlate their toxicity to their chemical composition, with the final aim of obtaining useful information for the agencies involved in the regulation of their components. |
| Databáze: | OpenAIRE |
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