Transcriptomic Analysis of Male Black Tiger Shrimp (Penaeus monodon) After Polychaete Feeding to Enhance Testicular Maturation
| Autor: | Umaporn Uawisetwathana, Rungnapa Leelatanawit, Suwanchai Phomklad, Jutatip Khudet, Somjai Wongtriphop, Pikul Jiravanichpaisal, Amornpan Klanchui, Nitsara Karoonuthaisiri |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Microarray Apoptosis Applied Microbiology and Biotechnology Penaeus monodon Andrology Transcriptome 03 medical and health sciences Vas Deferens Penaeidae Internal medicine Complementary DNA Gene expression Testis medicine Animals 030102 biochemistry & molecular biology biology Gene Expression Profiling Vas deferens Gene Expression Regulation Developmental Polychaeta DNA biology.organism_classification Animal Feed Shrimp 030104 developmental biology medicine.anatomical_structure Endocrinology Animal Nutritional Physiological Phenomena Spermatogenesis |
| Zdroj: | Marine biotechnology (New York, N.Y.). 19(2) |
| ISSN: | 1436-2236 |
| Popis: | To reveal molecular mechanism of how polychaetes enhanced reproductive maturation in the male black tiger shrimp (Penaeus monodon), transcriptomic profiles of male reproductive organs (testes and vas deferens) between polychaete-fed and commercial pellet-fed male brooders were compared using cDNA microarray. The overall profiles were distinguishingly different between the two feed groups as well as between testes and vas deferens. Additionally, six of 11 differentially expressed gene identified by the microarray (HNRPUL1 and GCP4 in testes, MAT2B, CDC16, and CSN5 in vas deferens, and SLD5 in both organs) were validated by quantitative real-time PCR (qPCR) and found to exhibit significantly higher expression levels in polychaete-fed shrimp than those in commercial pellet-fed shrimp. From microarray and qPCR results, the differentially expressed transcripts in both testes and vas deferens between different feeds belonged to DNA replication and microtubule nucleation pathways. Interestingly, while the transcripts involved in nutrient uptake and nucleotide biosynthesis were increased only in testes, those involved in protein refolding and apoptosis were increased only in vas deferens. These findings suggest that polychaetes may enhance spermatogenesis by increasing spermatogonia proliferation in testes and by regulating mature spermatozoa in vas deferens. |
| Databáze: | OpenAIRE |
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