Combination of interleukin-10 gene promoter polymorphisms with HLA-DRB1*15 allele is associated with multiple sclerosis
| Autor: | Maryam Koochaki, Hosein Amiri, Javad Sadeghi Allah Abadi, Rahim Kohansal, Danial Roshandel, Seied Mohammad Baghbanian, Mahdi Zamani, Majid Shahbazi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics lcsh:R lcsh:Medicine Promoter Single-nucleotide polymorphism General Medicine Biology General Biochemistry Genetics and Molecular Biology 3. Good health Histocompatibility 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Immunology Genotype Cytokine - HLA-DRB1*15 allele - interleukin-10 - multiple sclerosis - single nucleotide polymorphism Allele HLA-DRB1 Allele frequency Genotyping 030217 neurology & neurosurgery |
| Zdroj: | Indian Journal of Medical Research, Vol 145, Iss 6, Pp 746-752 (2017) |
| ISSN: | 0971-5916 |
| Popis: | Background & objectives: Multiple sclerosis (MS) is common in some ethnic groups. Interleukin-10 (IL-10) is a potent anti-inflammatory and immunosuppressive cytokine that may be an important regulator in MS disease pathogenesis. IL-10 promoter includes several single nucleotide polymorphisms and the level of IL-10 expression is related to these polymorphisms. Furthermore, loci within the histocompatibility regions are responsible for susceptibility to MS. The aim of this study was to investigate the association of IL-10 gene promoter polymorphisms and HLA-DRB1*15 allele frequencies with MS susceptibility in an Iranian population. Methods: In this study 336 MS patients and 454 healthy controls were included. Genomic DNA was purified from peripheral blood samples by a standard protocol. Genotyping was performed by the sequence-specific primer polymerase chain reaction method. Results: IL-10 −1082 G/G and IL-10 −819 C/C genotypes were more frequent in MS patients than healthy individuals. DRB1*15 allele showed a higher frequency among MS patients compared to controls. Interpretation & conclusions: The IL-10 and HLA-DRB1*15 polymorphisms were associated with the susceptibility to MS in Iranian patients. Our results suggest that gene-gene interaction of IL-10 polymorphisms and HLA-DRB1*15 alleles may be important factors in the development of MS. |
| Databáze: | OpenAIRE |
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