Detection of Rotavirus RNA and Antigens in Serum and Cerebrospinal Fluid Samples from Diarrheic Children with Seizures

Autor:	Bisei, Liu, Yukihiko, Fujita, Chikako, Arakawa, Ryutaro, Kohira, Tatsuo, Fuchigami, Hideo, Mugishima, Mitsutaka, Kuzuya
Rok vydání:	2009
Předmět:	Diarrhea Male Rotavirus Microbiology (medical) Genotype Reverse Transcriptase Polymerase Chain Reaction Infant Enzyme-Linked Immunosorbent Assay General Medicine Rotavirus Infections Feces Infectious Diseases Seizures Child Preschool Humans RNA Viral Capsid Proteins Female Antigens Viral
Zdroj:	Japanese Journal of Infectious Diseases. 62:279-283
ISSN:	1884-2836 1344-6304
DOI:	10.7883/yoken.jjid.2009.279
Popis:	Group A rotavirus (GARV) genes (the VP7 and NSP3 genes) in acute-phase cerebrospinal fluid (CSF), sera and stool samples from 6 children with convulsions accompanied by GARV gastroenteritis were investigated by reverse transcription-polymerase chain reaction (RT-PCR). When the VP7 gene was amplified from the samples, the G genotype (G type) of GARV was determined by RT-PCR. GARV genes were detected in the CSF samples of all 6 children, in 2 of the 3 blood samples, and in all of 4 stool samples. The G typing of GARV from 12 of a total of 13 samples indicated that G3 was the predominant G type in all samples. GARV antigens were detected by enzyme-linked immunosorbent assay in all of the 3 tested sera samples, while no GARV antigens were detected in any of the 5 tested CSF samples. We confirmed the presence of GARV genomes in the CSF samples from all of the children with rotavirus-associated seizures, including encephalopathy. However, the relationship between convulsions and the existence of GARV RNA in CSF remains unclear and further study is required.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbd9bb9f04a1fb773e8bc2b29dfa332d https://doi.org/10.7883/yoken.jjid.2009.279 Zobrazit plný text záznamu