Occurrence of pseudomonas spp. In raw vegetables: Molecular and phenotypical analysis of their antimicrobial resistance and virulence-related traits
| Autor: | Gabriela Chichón, María López, Carmen Torres, Lidia Ruiz-Roldán, Beatriz Rojo-Bezares, Yolanda Sáenz, Carmen Lozano |
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| Rok vydání: | 2021 |
| Předmět: |
Serotype
ST155 QH301-705.5 Virulence Biology biofilm Catalysis Microbiology Inorganic Chemistry chemistry.chemical_compound Antibiotic resistance pigment Biology (General) Physical and Theoretical Chemistry Insertion sequence QD1-999 Molecular Biology Gene Spectroscopy Organic Chemistry Pseudomonas Biofilm Rhamnolipid General Medicine OprD biology.organism_classification Computer Science Applications virulence Chemistry chemistry lasR |
| Zdroj: | RIUR. Repositorio Institucional de la Universidad de La Rioja instname International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 12626 International Journal of Molecular Sciences, Vol 22, Iss 12626, p 12626 (2021) |
| Popis: | Pseudomonas is characterized by its great capacity to colonize different ecological niches, but also by its antimicrobial resistance and pathogenicity, causing human, animal, or plant diseases. Raw and undercooked food is a potential carrier of foodborne disease. The aim of this study was to determine the occurrence of Pseudomonas spp. among raw vegetables, analysing their antimicrobial resistance, virulence, and molecular typing. A total of 163 Pseudomonas spp. isolates (12 different species) were recovered from 77 of the 145 analysed samples (53.1%) and were classified into 139 different pulsed-field gel electrophoresis patterns. Low antimicrobial resistance levels, but one multidrug-resistant isolate, were found. Among the 37 recovered P. aeruginosa strains, 28 sequence-types and nine serotypes were detected. Eleven OprD patterns and an insertion sequence (ISPa1635) truncating the oprD gene of one imipenem-resistant strain were found. Ten virulotypes were observed, including four exoU-positive and thirty-one exoS-positive strains. The lasR gene was absent in three ST155 strains and was truncated by different insertion sequences (ISPre2, IS1411, and ISPst7) in other three strains. High biofilm, motility, pigment, elastase, and rhamnolipid production were detected. Our study demonstrated a low occurrence of P. aeruginosa (18%) and low antimicrobial resistance, but a high number of virulence-related traits in these P. aeruginosa strains, highlighting their pathological importance. |
| Databáze: | OpenAIRE |
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