Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families
| Autor: | Ricardo Flores, Angel Emilio Martínez de Alba, Selma Gago, Alberto Carbonell |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Ribonuclease III
Small interfering RNA RNA-induced transcriptional silencing Chrysanthemum Viroid RNA-induced silencing complex viruses Pospiviroidae Molecular Sequence Data Trans-acting siRNA Asteraceae Infections Solanum lycopersicum Virology RNA-Induced Silencing Complex RNA Processing Post-Transcriptional RNA Small Interfering Plant Diseases RNA Double-Stranded Genetics Base Sequence biology RISC RNA RNA Circular Argonaute Plants Genetically Modified biology.organism_classification Viroids RNA silencing RNA Plant Avsunviroidae RNA Viral RNA Interference Dicer |
| Zdroj: | Virology. 371:44-53 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2007.09.031 |
| Popis: | Infection by viroids, non-protein-coding circular RNAs, occurs with the accumulation of 21–24 nt viroid-derived small RNAs (vd-sRNAs) with characteristic properties of small interfering RNAs (siRNAs) associated to RNA silencing. The vd-sRNAs most likely derive from dicer-like (DCL) enzymes acting on viroid-specific dsRNA, the key elicitor of RNA silencing, or on the highly structured genomic RNA. Previously, viral dsRNAs delivered mechanically or agroinoculated have been shown to interfere with virus infection in a sequence-specific manner. Here, we report similar results with members of the two families of nuclear- and chloroplast-replicating viroids. Moreover, homologous vd-sRNAs co-delivered mechanically also interfered with one of the viroids examined. The interference was sequence-specific, temperature-dependent and, in some cases, also dependent on the dose of the co-inoculated dsRNA or vd-sRNAs. The sequence-specific nature of these effects suggests the involvement of the RNA induced silencing complex (RISC), which provides sequence specificity to RNA silencing machinery. Therefore, viroid titer in natural infections might be regulated by the concerted action of DCL and RISC. Viroids could have evolved their secondary structure as a compromise between resistance to DCL and RISC, which act preferentially against RNAs with compact and relaxed secondary structures, respectively. In addition, compartmentation, association with proteins or active replication might also help viroids to elude their host RNA silencing machinery. |
| Databáze: | OpenAIRE |
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