New insights into sequence recognition process of esperamicin A1 and calicheamicin .gamma.1I: Origin of their selectivities and 'induced fit' mechanism
| Autor: | Yukio Sugiura, Motonari Uesugi |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Esperamicin
Circular dichroism Conformational change Antibiotics Antineoplastic Binding Sites Base Sequence Stereochemistry Circular Dichroism Esperamicin A1 Molecular Sequence Data DNA Biochemistry Anti-Bacterial Agents chemistry.chemical_compound Aminoglycosides chemistry Calicheamicin Enediyne Nucleic Acid Conformation Calicheamicin Gamma 1I Enediynes |
| Zdroj: | Biochemistry. 32:4622-4627 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi00068a020 |
| Popis: | This study addresses the DNA sequence recognition event of the enediyne antibiotics esperamicin A1 and calicheamicin gamma 1I by the use of synthetic DNA oligomers, salt effects, and circular dichroism studies. The results reported here provide several important insights: (1) esperamicin A1 requires a purine/pyrimidine trimer in host DNA for favorable interaction, (2) the sequence selectivity of esperamicin C is an origin of esperamicin A1 and calicheamicin gamma 1I selectivities, (3) in the target recognition by esperamicin C, its total structure and hydrophobicity are important, and (4) the binding of hydrophobic esperamicin to DNA duplex induces dehydration and conformational change of the host DNA. The specific sequence recognition process of esperamicin/calicheamicin has been discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|