The cyclic adenosine monophosphate elevating medicine, forskolin, reduces neointimal formation and atherogenesis in mice
| Autor: | Zekun Peng, Zhenyu Xu, Miao Wang, Yuze Zhang, Xiaoyan Ma, Chuansheng Xu, Liyuan Zhu, Huifeng Hao, Hong Chen, Qiaoling Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Neointima Endothelium endothelium Myocytes Smooth Muscle Inflammation Blood Pressure Pharmacology Muscle Smooth Vascular forskolin 03 medical and health sciences chemistry.chemical_compound restenosis Mice 0302 clinical medicine Restenosis cAMP medicine Cyclic AMP Leukocytes Animals Cyclic adenosine monophosphate Aorta Cell Proliferation Neointimal hyperplasia Forskolin Hyperplasia business.industry Colforsin Endothelial Cells Cell Biology Original Articles medicine.disease Atherosclerosis Endothelial stem cell Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Molecular Medicine Medicine Original Article medicine.symptom business Signal Transduction |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | Neointimal formation and atherogenesis are major vascular complications following percutaneous coronary intervention, and there is lack of pharmacological therapy. This study was aimed to examine the effect of forskolin (FSK), a cyclic adenosine monophosphate (cAMP)‐elevating agent, on vascular response to angioplasty wire injury and on atherogenesis in mice. Forskolin treatment reduced neointima formation at 7 and 28 days after wire injury. Early morphometrics of the injured vessels revealed that FSK treatment enhanced endothelial repair and reduced inflammatory cell infiltration. In vitro treatment of primary aortic cells with FSK, at 3‐100 μmol/L, increased endothelial cell proliferation, whereas FSK, at 30‐100 μmol/L, inhibited smooth muscle cell proliferation. FSK inhibited lipopolysaccharide‐induced leucocyte‐endothelial interaction in vitro and in vivo. In a mouse model of atherosclerosis driven by dyslipidaemia and hypertension, FSK administration increased endothelial repair and reduced atherosclerotic plaque formation, without affecting blood pressure, plasma lipids or aortic aneurysms formation. In summary, FSK, at doses relevant to human therapeutic use, protects against neointimal hyperplasia and atherogenesis, and this is attributable to its activities on pro‐endothelial repair and anti‐inflammation. This study raises a potential of clinical use of FSK as an adjunct therapy to prevent restenosis and atherosclerosis after percutaneous coronary intervention. |
| Databáze: | OpenAIRE |
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