Environment and Diet Influence the Bacterial Microbiome of Ambigolimax valentianus, an Invasive Slug in California

Autor:	Mia R. Maltz, Emma L. Aronson, Denise Jackson, James Borneman, Hannah L Freund
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0106 biological sciences 0301 basic medicine food.ingredient animal structures gastropod Slug Science Zoology microbiome Sterile diet Ambigolimax 010603 evolutionary biology 01 natural sciences 03 medical and health sciences food terrestrial slug Microbiome bacteria Nutrition biology Host (biology) fungi food and beverages biology.organism_classification 030104 developmental biology host Insect Science embryonic structures 16s rrna gene sequencing Biological dispersal PEST analysis
Zdroj:	Insects Volume 12 Issue 7 Insects, vol 12, iss 7 Insects, Vol 12, Iss 575, p 575 (2021)
ISSN:	2075-4450
DOI:	10.3390/insects12070575
Popis:	Ambigolimax valentianus is an invasive European terrestrial gastropod distributed throughout California. It is a serious pest of gardens, plant nurseries, and greenhouses. We evaluated the bacterial microbiome of whole slugs to capture a more detailed picture of bacterial diversity and composition in this host. We concentrated on the influences of diet and environment on the Ambigolimax valentianus core bacterial microbiome as a starting point for obtaining valuable information to aid in future slug microbiome studies. Ambigolimax valentianus were collected from two environments (gardens or reared from eggs in a laboratory). DNA from whole slugs were extracted and next-generation 16S rRNA gene sequencing was performed. Slug microbiomes differed between environmental sources (garden- vs. lab-reared) and were influenced by a sterile diet. Lab-reared slugs fed an unsterile diet harbored greater bacterial species than garden-reared slugs. A small core microbiome was present that was shared across all slug treatments. This is consistent with our hypothesis that a core microbiome is present and will not change due to these treatments. Findings from this study will help elucidate the impacts of slug-assisted bacterial dispersal on soils and plants, while providing valuable information about the slug microbiome for potential integrated pest research applications.
Databáze:	OpenAIRE
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