Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro

Autor: Mary Anne Roshni Amalaradjou, Sangeetha Ananda Baskaran, Kumar Venkitanarayanan, Amoolya Narayanan
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Skin Neoplasms
Colorectal cancer
Cell Cycle Proteins
lcsh:Chemistry
chemistry.chemical_compound
Nuclear Receptor Subfamily 4
Group A
Member 1
lcsh:QH301-705.5
Spectroscopy
Caspase 8
medium chain fatty acids
Goats
General Medicine
Up-Regulation
Computer Science Applications
anticarcinogenic properties
Milk
Biochemistry
Female
Caprylates
Colorectal Neoplasms
human colon cells
Cyclin-Dependent Kinase Inhibitor p21
Cell Survival
Down-Regulation
Antineoplastic Agents
Breast Neoplasms
Biology
Article
Catalysis
Caproic Acid
Inorganic Chemistry
Breast cancer
Cell Line
Tumor
medicine
Animals
Humans
Viability assay
Physical and Theoretical Chemistry
Caproates
Molecular Biology
Cell Proliferation
human breast cells
Organic Chemistry
Cancer
Decanoic acid
HCT116 Cells
medicine.disease
chemistry
lcsh:Biology (General)
lcsh:QD1-999
human skin cells
Cancer cell
Cancer research
Skin cancer
Decanoic Acids
Zdroj: International Journal of Molecular Sciences, Vol 16, Iss 3, Pp 5014-5027 (2015)
International Journal of Molecular Sciences
Volume 16
Issue 3
Pages 5014-5027
ISSN: 1422-0067
Popis: Colorectal cancer, breast cancer and skin cancer are commonly-reported cancer types in the U.S. Although radiation and chemotherapy are routinely used to treat cancer, they produce side effects in patients. Additionally, resistance to chemotherapeutic drugs has been noticed in cancers. Thus, there is a need for effective and safe bioprophylactics and biotherapeutics in cancer therapy. The medicinal value of goat milk has been recognized for centuries and is primarily attributed to three fatty acids, namely capric, caprylic and caproic acids. This research investigates the anticancer property of these fatty acids on human colorectal, skin and mammary gland cancer cells. The cancer cells were treated with various concentrations of fatty acids for 48 h, and cell viability was monitored by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. Additionally, real-time quantitative PCR (RT-qPCR) was performed to elucidate the potential anti-cancer mechanisms of the three fatty acids under investigation. Capric, caprylic and caproic acids reduced cancer cell viability by 70% to 90% (p <
0.05) compared to controls. RT-qPCR data indicated that these natural molecules produced anticancer effects by down-regulating cell cycle regulatory genes and up-regulating genes involved in apoptosis. Future research will validate the anticancer effect of these fatty acids in an appropriate in vivo model.
Databáze: OpenAIRE
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