Evidence for Transcriptional Induction of the Liver Fatty-Acid-Binding-Protein Gene by Bezafibrate in the Small Intestine

Autor: Hélène Poirier, A. Mallordy, Isabelle Niot, Hélène Carlier, Philippe Besnard
Přispěvatelé: Laboratoire de Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)
Jazyk: angličtina
Rok vydání: 1995
Předmět:
Male
Transcription
Genetic
[SDV]Life Sciences [q-bio]
Biochemistry
chemistry.chemical_compound
Mice
0302 clinical medicine
Cytosol
Gene expression
Intestine
Small
Transcriptional regulation
Sunflower Oil
transcriptional regulation
Cycloheximide
0303 health sciences
bezafibrate
030302 biochemistry & molecular biology
Fatty Acids
Fatty‐acid‐binding protein (FABPc)
Neoplasm Proteins
medicine.anatomical_structure
Liver
Fatty Acid-Binding Protein 7
medicine.drug
medicine.medical_specialty
Ileum
Nerve Tissue Proteins
Biology
Fatty Acid-Binding Proteins
lipids
03 medical and health sciences
Internal medicine
medicine
Animals
Plant Oils
RNA
Messenger
030304 developmental biology
Messenger RNA
Bezafibrate
Small intestine
Endocrinology
chemistry
Gene Expression Regulation
Duodenum
Carrier Proteins
small intestine
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
030217 neurology & neurosurgery
Zdroj: European Journal of Biochemistry
European Journal of Biochemistry, Wiley, 1995, 227 (3), pp.801-807. ⟨10.1111/j.1432-1033.1995.0801p.x⟩
ISSN: 0014-2956
1432-1327
DOI: 10.1111/j.1432-1033.1995.0801p.x⟩
Popis: International audience; The effect of bezafibrate on cytosolic fatty‐acid‐binding‐protein (FABPc) production along the small intestine has been investigated in mice. This drug increased the intestinal fatty‐acid‐binding‐protein (I‐FABPc) and liver fatty‐acid‐binding‐protein (L‐FABPc) mRNA levels in the duodenum. The extents of induction in the duodenum and in the liver are similar. However, the degree of stimulation gradually decreases along the length of the gut, no effect being found in the ileum. An efficient absorption of this drug as early as the proximal part of the small intestine may explain this phenomenon. The L‐FABPc gene is silent in terminal ileum of mice, but a direct infusion of bezafibrate into the ileum switches it on. We used this original model to follow the time course of induction of the L‐FABPc gene by bezafibrate. L‐FABPc mRNA was first detected 4 h after fibrate infusion, reached a maximum level at 16 h and subsequently decreased at 24 h. This induction was totally blocked by cycloheximide. Sunflower oil also caused small increases in the L‐FABPc mRNA levels. The transcriptional origin of the induction triggered both by bezafibrate and sunflower oil was demonstrated by run‐on assays. These data indicate that (a) the transcription of the L‐FABPc gene is induced by bezafibrate via de novo protein synthesis and (b) components of sunflower oil can transcriptionally activate the L‐FABPc gene. Our results also demonstrate that the mouse terminal ileum is a useful system for studying the regulation of L‐FABPc gene expression both in vivo and in vitro.
Databáze: OpenAIRE
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