'Activography': a novel, versatile and easily adaptable method for monitoring enzymatic activities in situ

Autor:	Kostas Vekrellis, Georgios Pampalakis, Eleni Zingkou, Georgia Sotiropoulou
Rok vydání:	2017
Předmět:	0301 basic medicine In situ Organophosphonates Biotin Nanotechnology 01 natural sciences Catalysis Mice 03 medical and health sciences In situ zymography Materials Chemistry Animals Skin Mice Knockout Molecular Structure 010405 organic chemistry Chemistry Metals and Alloys General Chemistry 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials 030104 developmental biology Tissue sections Molecular Probes Ceramics and Composites Serine Peptidase Inhibitor Kazal-Type 5 Kallikreins Biomedical engineering
Zdroj:	Chemical Communications. 53:3246-3248
ISSN:	1364-548X 1359-7345
DOI:	10.1039/c7cc01081h
Popis:	We developed activography to map enzymatic activities on tissue sections using activity-based probes. The assay was validated using a new protease-activity probe on skin biopsies to provide proof-of-concept. Activography is more selective and technically easier than the established in situ zymography, thus, adaptable in routine running clinico-chemical laboratories.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbc8acd04a8b27e579ad62160955ab8a https://doi.org/10.1039/c7cc01081h Zobrazit plný text záznamu