PDX regulates inflammatory cell infiltration via resident macrophage in LPS-induced lung injury
| Autor: | Shu-Yang Xiang, Fang Gao Smith, Qian Yang, Shengwei Jin, Yang Ye, Hao-Ran Xu, Zhang Huawei, Shengxing Zheng, Hong-Xia Mei, Qian Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Docosahexaenoic Acids Neutrophils Receptors CCR2 Acute Lung Injury Chemokine CXCL2 Endogeny Lung injury Pharmacology MMP9 Receptors Interleukin-8B Flow cytometry Pathogenesis 03 medical and health sciences Chemokine receptor Mice 0302 clinical medicine In vivo medicine Animals Administration Intranasal Chemokine CCL2 Inflammation medicine.diagnostic_test Chemistry Tumor Necrosis Factor-alpha Macrophages Transendothelial and Transepithelial Migration Cell Biology medicine.disease Mice Inbred C57BL Chemotaxis Leukocyte 030104 developmental biology Matrix Metalloproteinase 9 030220 oncology & carcinogenesis Liposomes Molecular Medicine Clodronic Acid Infiltration (medical) Injections Intraperitoneal Signal Transduction |
| Zdroj: | Journal of cellular and molecular medicine. 24(18) |
| ISSN: | 1582-4934 |
| Popis: | Inflammatory cell infiltration contributes to the pathogenesis of acute respiratory distress syndrome (ARDS). Protectin DX (PDX), an endogenous lipid mediator, shows anti-inflammatory and proresolution bioactions. In vivo, the mice were intraperitoneally injected with PDX (0.1 µg/mouse) after intratracheal (1 mg/kg) or intraperitoneal (10 mg/kg) LPS administration. Flow cytometry was used to measure inflammatory cell numbers. Clodronate liposomes were used to deplete resident macrophages. RT-PCR, and ELISA was used to measure MIP-2, MCP-1, TNF-α and MMP9 levels. In vitro, sorted neutrophils, resident and recruited macrophages (1 × 106 ) were cultured with 1 μg/mL LPS and/or 100 nmol/L PDX to assess the chemokine receptor expression. PDX attenuated LPS-induced lung injury via inhibiting recruited macrophage and neutrophil recruitment through repressing resident macrophage MCP-1, MIP-2 expression and release, respectively. Finally, PDX inhibition of neutrophil infiltration and transmembrane was associated with TNF-α/MIP-2/MMP9 signalling pathway. These data suggest that PDX attenuates LPS-stimulated lung injury via reduction of the inflammatory cell recruitment mediated via resident macrophages. |
| Databáze: | OpenAIRE |
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