v-SNARE cellubrevin is required for basolateral sorting of AP-1B-dependent cargo in polarized epithelial cells
| Autor: | Ian C. Fields, Heike Fölsch, Véronique Proux-Gillardeaux, Marc Pypaert, Thierry Galli, Elina Shteyn, Richard S. Kang |
|---|---|
| Přispěvatelé: | Department of Biochemistry, Molecular Biology & Cell Biology Northwestern University, Department of Biochemistry, Molecular Biology & Cell Biology-Northwestern University, Department of Cell Biology [New Haven], Yale University School of Medicine-Howard Hughes Medical Institute (HHMI), Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (Avenir Program), the European Commission ('Signaling and Traffic' STREP 503229), the Association pour la Recherche sur le Cancer, the Ministère de la Recherche (ACI-BDP), the Fondation pour la Recherche Médicale, Yale University School of Medicine-Howard Hugues Medical Institute |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Vesicle-Associated Membrane Protein 3
Endosome Adaptor Protein Complex 1 Endosomes [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Membrane Fusion Article Cell Line 03 medical and health sciences Dogs 0302 clinical medicine Tetanus Toxin Cell polarity Animals Humans Adaptor Protein Complex beta Subunits [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Integral membrane protein Research Articles 030304 developmental biology Epithelial polarity 0303 health sciences Qa-SNARE Proteins Cell Membrane Cell Polarity Metalloendopeptidases Lipid bilayer fusion Epithelial Cells Cell Biology Basolateral plasma membrane Transmembrane protein Transport protein Cell biology Protein Transport Receptors LDL SNARE Proteins 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cell Biology Journal of Cell Biology, Rockefeller University Press, 2007, 177 (3), pp.477-88. ⟨10.1083/jcb.200610047⟩ The Journal of Cell Biology |
| ISSN: | 0021-9525 1540-8140 |
| Popis: | International audience; The epithelial cell-specific adaptor complex AP-1B is crucial for correct delivery of many transmembrane proteins from recycling endosomes to the basolateral plasma membrane. Subsequently, membrane fusion is dependent on the formation of complexes between SNARE proteins located at the target membrane and on transport vesicles. Although the t-SNARE syntaxin 4 has been localized to the basolateral membrane, the v-SNARE operative in the AP-1B pathway remained unknown. We show that the ubiquitously expressed v-SNARE cellubrevin localizes to the basolateral membrane and to recycling endosomes, where it colocalizes with AP-1B. Furthermore, we demonstrate that cellubrevin coimmunoprecipitates preferentially with syntaxin 4, implicating this v-SNARE in basolateral fusion events. Cleavage of cellubrevin with tetanus neurotoxin (TeNT) results in scattering of AP-1B localization and missorting of AP-1B-dependent cargos, such as transferrin receptor and a truncated low-density lipoprotein receptor, LDLR-CT27. These data suggest that cellubrevin and AP-1B cooperate in basolateral membrane trafficking. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|