MicroRNA-595 sensitizes ovarian cancer cells to cisplatin by targeting ABCB1
| Autor: | Mingyue Zhang, Songyu Tian, Xiuwei Chen, Ge Lou, Yunduo Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology endocrine system medicine.medical_specialty ATP Binding Cassette Transporter Subfamily B endocrine system diseases Cell Antineoplastic Agents miR-595 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Internal medicine microRNA medicine Humans Genes Tumor Suppressor Neoplasm Invasiveness Lymph node Cell Proliferation Ovarian Neoplasms Cisplatin Cell growth business.industry Cancer ABCB1 medicine.disease female genital diseases and pregnancy complications microRNAs Gene Expression Regulation Neoplastic ovarian cancer 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Female Ectopic expression Ovarian cancer business Research Paper medicine.drug |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.13526 |
| Popis: | // Songyu Tian 1 , Mingyue Zhang 2 , Xiuwei Chen 1 , Yunduo Liu 1 , Ge Lou 1 1 Department of Gynecology Oncology, Cancer Hospital of Harbin Medical University, Harin, 150081, Heilongjiang, China 2 Department of Anaesthesiology, Cancer Hospital of Harbin Medical University, Harin, 150081, Heilongjiang, China Correspondence to: Ge Lou, email: louge16@tom.com Keywords: ovarian cancer, microRNAs, miR-595, ABCB1 Received: July 25, 2016 Accepted: November 09, 2016 Published: November 23, 2016 ABSTRACT Ovarian cancer is among the leading cause of cancer-related deaths in females. In this study, we demonstrated that miR-595 expression was downregulated in the ovarian cancer tissues and cell lines. miR-595 expression was lower in the lymph node metastases tissues than in the primary ovarian cancer tissues and normal tissues. Furthermore, miR-595 overexpression suppressed the ovarian cancer cell proliferation, colony formation and invasion and promoted the sensitivity of ovarian cancer cell to cisplatin. We identified ABCB1 as a direct target gene of miR-595 in the ovarian cancer cell. ABCB1 expression was upregulated in the ovarian cancer tissues and cell lines. Morevoer, the expression level of ABCB1 was inversely correlated with miR-595 in the ovarian cancer tissues. In addition, overexpression of ABCB1 decreased the miR-595-overexpressing HO8910PM and SKOV-3 cell sensitivity to cisplatin. Ectopic expression of ABCB1 promoted the miR-595-overexpressing HO8910PM and SKOV-3 cell proliferation, colony formation and invasion. These data suggested that miR-595 acted a tumor suppressor role in ovarian cancer development and increased the sensitivity of ovarian cancer to cisplatin. |
| Databáze: | OpenAIRE |
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