Proteomic analysis of rat aorta during atherosclerosis induced by high cholesterol diet and injection of vitamin D3
| Autor: | Mohamad Radwan Almofti, Zhenyu Huang, Pengyuan Yang, Yaocheng Rui |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Vitamin
Male Proteomics Physiology Mass spectrometry Peptide Mapping Cholesterol Dietary chemistry.chemical_compound Physiology (medical) medicine.artery Databases Genetic medicine Animals Electrophoresis Gel Two-Dimensional Rats Wistar Aorta Cholecalciferol Pharmacology chemistry.chemical_classification biology Aldolase A NADH dehydrogenase Vitamins Atherosclerosis Molecular biology Rats Enzyme Biochemistry chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Diet Atherogenic Indicators and Reagents Lamin Biomarkers |
| Zdroj: | Clinical and experimental pharmacologyphysiology. 33(4) |
| ISSN: | 0305-1870 |
| Popis: | SUMMARY 1 Atherosclerosis (AS) in rats displays important clinical similarities to human AS. 2 After the experimental model of AS in rat was established and using a proteomic approach, we compared the protein profiling of aorta tissues from healthy and AS rats. 3 Using two-dimensional electrophoresis (2-DE), over 1878 protein species were separated; among them, 1239 protein spots were matched between different gels with average matching rate of approximately 66%. Gel analysis and protein characterization have identified 58 protein spots whose abundance is significantly altered in AS rats. 4 By using matrix-associated laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) and NCBInr database, 46 proteins were successfully identified. Among them, 18 proteins were of increased abundance in diseased tissues including a group of oxidization-related enzymes such as peroxiredoxin2 and NADH dehydrogenase Fe-S protein 6, components of inflammatory pathways such as lamin A, while 28 proteins were of decreased abundance in the diseased state, including CaM-KII inhibitory protein, transferring, fructose-bisphosphate aldolase. 5 We believe that these results would give insights into the cellular and molecular mechanisms involved in AS development and might lead to the discovery of novel diagnostic markers and new therapeutic opportunities. |
| Databáze: | OpenAIRE |
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