Identification and characterization of miRNAs involved in cold acclimation of zebrafish ZF4 cells
| Autor: | Yajing Bai, Xiangqin Ji, Junfang Zhang, Mengjia Li, Juntao Luo, Bingshe Han, Penglei Jiang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
cDNA libraries
0301 basic medicine Molecular biology Acclimatization Biochemistry Sequencing techniques 0302 clinical medicine Gene expression DNA libraries Zebrafish Regulation of gene expression Multidisciplinary Small nuclear RNA biology MRNA cleavage Physics High-Throughput Nucleotide Sequencing Eukaryota Classical Mechanics RNA sequencing Animal Models Complementary DNA Cell biology Cold Temperature Nucleic acids Intracellular signal transduction Experimental Organism Systems Small nucleolar RNA Osteichthyes 030220 oncology & carcinogenesis Vertebrates Physical Sciences Medicine Mechanical Stress Research Article Forms of DNA Science Research and Analysis Methods Cell Line 03 medical and health sciences Model Organisms Extraction techniques Genetics Cold acclimation Animals KEGG Non-coding RNA Natural antisense transcripts Biology and life sciences Sequence Analysis RNA Gene Expression Profiling Organisms DNA biology.organism_classification RNA extraction Gene regulation Gene expression profiling MicroRNAs Fish Thermal Stresses Molecular biology techniques 030104 developmental biology Animal Studies RNA |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 1, p e0226905 (2020) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0226905 |
| Popis: | MicroRNAs (miRNAs) play vital roles in various biological processes under multiple stress conditions by leading to mRNA cleavage or translational repression. However, the detailed roles of miRNAs in cold acclimation in fish are still unclear. In the present study, high-throughput sequencing was performed to identify miRNAs from 6 small RNA libraries from the zebrafish embryonic fibroblast ZF4 cells under control (28°C, 30 days) and cold-acclimation (18°C, 30 days) conditions. A total of 414 miRNAs, 349 known and 65 novel, were identified. Among those miRNAs, 24 (19 known and 5 novel) were up-regulated, and 23 (9 known and 14 novel) were down-regulated in cold acclimated cells. The Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analyses indicated that the target genes of known differentially expressed miRNAs (DE-miRNA) are involved in cold acclimation by regulation of phosphorylation, cell junction, intracellular signal transduction, ECM-receptor interaction and so on. Moreover, both miR-100-3p inhibitor and miR-16b mimics could protect ZF4 cells under cold stress, indicating the involvement of miRNA in cold acclimation. Further study showed that miR-100-3p and miR-16b could regulate inversely the expression of their target gene (atad5a, cyp2ae1, lamp1, rilp, atxn7, tnika, btbd9), and that overexpression of miR-100-3p disturbed the early embryonic development of zebrafish. In summary, the present data show that miRNAs are closely involved in cold acclimation in zebrafish ZF4 cells and provide information for further understanding of the roles of miRNAs in cold acclimation in fish. |
| Databáze: | OpenAIRE |
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