DNA methylation and gene expression regulation associated with vascularization in Sorghum bicolor
| Autor: | Denis Tolkunov, Doreen Ware, Andrew Olson, Govindarajan Kunde-Ramamoorthy, Barbara M. Waring, Shweta Deshphande, Gina Turco, J. Peter Etchells, Chew Yee Ngan, Siobhan M. Brady, Chia-Lin Wei, Jeremy Schmutz, Shawn M. Kaeppler, Patricia E. Klein, Scott C. Stelpflug, Kaisa Kajala |
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| Přispěvatelé: | Sub Ecology and Biodiversity, Ecology and Biodiversity |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology Plant Biology & Botany Plant Science Biology Genes Plant Plant Roots Transcriptome 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Cell Wall Gene expression Genetics Epigenetics Gene Sorghum Conserved Sequence Regulation of gene expression Full Paper epigenetics Agricultural and Veterinary Sciences Research Human Genome food and beverages Plant Methylation Full Papers DNA Methylation Biological Sciences sorghum (Sorghum bicolor) cell type-specific cell type‐specific 030104 developmental biology Gene Expression Regulation Genes chemistry DNA methylation biofuel sorghum Plant Vascular Bundle DNA |
| Zdroj: | The New phytologist, vol 214, iss 3 The New Phytologist New phytologist., 2017, Vol.214(3), pp.1213-1229 [Peer Reviewed Journal] New Phytologist, 214(3), 1213. Blackwell Publishing Ltd |
| ISSN: | 1469-8137 0028-646X |
| Popis: | Summary Plant secondary cell walls constitute the majority of plant biomass. They are predominantly found in xylem cells, which are derived from vascular initials during vascularization. Little is known about these processes in grass species despite their emerging importance as biomass feedstocks. The targeted biofuel crop Sorghum bicolor has a sequenced and well‐annotated genome, making it an ideal monocot model for addressing vascularization and biomass deposition.Here we generated tissue‐specific transcriptome and DNA methylome data from sorghum shoots, roots and developing root vascular and nonvascular tissues.Many genes associated with vascular development in other species show enriched expression in developing vasculature. However, several transcription factor families varied in vascular expression in sorghum compared with Arabidopsis and maize. Furthermore, differential expression of genes associated with DNA methylation were identified between vascular and nonvascular tissues, implying that changes in DNA methylation are a feature of sorghum root vascularization, which we confirmed using tissue‐specific DNA methylome data. Roots treated with a DNA methylation inhibitor also showed a significant decrease in root length.Tissues and organs can be discriminated based on their genomic methylation patterns and methylation context. Consequently, tissue‐specific changes in DNA methylation are part of the normal developmental process. |
| Databáze: | OpenAIRE |
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