Effects of mutation of 2,3-butanediol formation pathway on glycerol metabolism and 1,3-propanediol production by Klebsiella pneumoniae J2B
| Autor: | Doyoung Seung, Vinod Kumar, Meetu Durgapal, Ashok Somasundar, Chelladurai Rathnasingh, Hyohak Song, Mugesh Sankaranarayanan, Sunghoon Park |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Glycerol Environmental Engineering Mutant Glycerol dehydratase ΔbudO mutant Bioengineering Biology 03 medical and health sciences chemistry.chemical_compound Klebsiella pneumoniae J2B Bioreactors Lactate dehydrogenase 2 3-Butanediol Glycolysis 1 3-Propanediol Butylene Glycols Waste Management and Disposal Renewable Energy Sustainability and the Environment General Medicine Metabolism Biosynthetic Pathways Klebsiella pneumoniae 030104 developmental biology chemistry Biochemistry Propylene Glycols Mutation |
| Popis: | The current study investigates the impact of mutation of 2,3-butanediol (BDO) formation pathway on glycerol metabolism and 1,3-propanediol (PDO) production by lactate dehydrogenase deficient mutant of Klebsiella pneumoniae J2B. To this end, BDO pathway genes, budA, budB, budC and budO (whole-bud operon), were deleted from K. pneumoniae J2B ΔldhA and the mutants were studied for glycerol metabolism and alcohols (PDO, BDO) production. ΔbudO-mutant-only could completely abolish BDO production, but with reductions in cell growth and PDO production. By modifying the culture medium, the ΔbudO mutant could recover its performance on the flask scale. However, in bioreactor experiments, the ΔbudO mutant accumulated a significant amount of pyruvate (>73 mM) in the late phase and PDO production stopped concomitantly. Glycolytic intermediates of glycerol, especially glyceraldehyde-3-phosphate (G3P) was highly inhibitory to glycerol dehydratase (GDHt); its accumulation, followed by pyruvate accumulation, was assumed to be responsible for the ΔbudO mutant’s low PDO production. |
| Databáze: | OpenAIRE |
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