Retention of ribosomal ribonucleic acids in agarose gels
| Autor: | Milka B. Novaković, S.L. Petrovic, Jelena Petrović |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Macromolecular Substances
Sodium Chloride Biochemistry Genetics and Molecular Biology (miscellaneous) chemistry.chemical_compound Polysaccharides Centrifugation Density Gradient Escherichia coli Animals Binding Sites Chromatography Nacl solutions Osmolar Concentration Temperature Phosphorus Isotopes RNA Cations Monovalent Plants Ribosomal RNA Alkali metal Rats Molecular Weight Liver chemistry RNA Ribosomal Ionic strength Rat liver Chromatography Gel Agarose Selectivity Gels |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 308:317-323 |
| ISSN: | 0005-2787 |
| DOI: | 10.1016/0005-2787(73)90161-5 |
| Popis: | Ribosomal RNAs from various organisms could be immobilized in agarose gels at high ionic strength in a thermally reversible fashion. In NaCl solutions at 23 °C, agarose capacity for retention of liver rRNAs was about 0.4 mg/ml gel under saturating conditions. NaCl molarities necessary for a 50 % retention of rat liver rRNAs increase with temperature (in the range of 23–37 °C) by 0.04–0.05 M/degree for the larger (28-S) component, and by 0.07–0.09 M/degree for the smaller (18-S) component. The retention process also displays cation selectivity; for rat liver 28-S RNA, molarities of alkali metal chlorides at 50 % retention and 23 °C were: LiCl and NaCl, about 0.4 M; KCl, about 0.6 M; CsCl, about 0.9 M. The retention molarities of NaCl tend to decrease with increase in both the molecular weight and the G + C content of rRNAs, but the relationship of these variables is clearly non-linear, and it might depend on undetermined features of rRNA structure. The retention profiles appear to be largely characteristic of respective species of rRNAs. |
| Databáze: | OpenAIRE |
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