Pectate hydrolases of parsley (Petroselinum crispum) roots
| Autor: | Zdenek Voburka, Dana Flodrová, Danica Mislovičová, Fairouz Ait Mohand, Eva Stratilová, Mária Dzúrová, Desana Lišková, Anna Malovícová, Jirina Omelkova |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
Chromatography Glycoside Hydrolases Ph optimum Chemistry Isoelectric focusing A protein Petroselinum crispum Hydrogen-Ion Concentration Solid medium Plant Roots General Biochemistry Genetics and Molecular Biology Mixed Function Oxygenases Kinetics Mice Enzyme Polymerization Animals Electrophoresis Polyacrylamide Gel Petroselinum Isoelectric Focusing Lower degree Polysaccharide-Lyases |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0939-5075 |
| Popis: | The presence of various enzyme forms with terminal action pattern on pectate was evaluated in a protein mixture obtained from parsley roots. Enzymes found in the soluble fraction of roots (juice) were purified to homogeneity according to SDS-PAGE, partially separated by preparative isoelectric focusing and characterized. Three forms with pH optima 3.6, 4.2 and 4.6 clearly preferred substrates with a lower degree of polymerization (oligogalacturonates) while the form with pH optimum 5.2 was a typical exopolygalacturonase [EC 3. 2.1.67] with relatively fast cleavage of polymeric substrate. The forms with pH optima 3.6, 4.2 and 5.2 were released from the pulp, too. The form from the pulp with pH optimum 4.6 preferred higher oligogalacturonates and was not described in plants previously. The production of individual forms in roots was compared with that produced by root cells cultivated on solid medium and in liquid one. |
| Databáze: | OpenAIRE |
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