Extensive Evaluation via Blinded Testing of an UHPLC-MS/MS Method for Quantitation of Ten Ergot Alkaloids in Rye and Wheat Grains
| Autor: | Jake Guag, Kelly Benson, Renate Reimschuessel, Michelle S. Mostrom, Brett T. Webb, Andriy Tkachenko |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Ergot Alkaloids Routine testing 01 natural sciences Uhplc ms ms Claviceps Method test Analytical Chemistry Food and drug administration 03 medical and health sciences Tandem Mass Spectrometry Ergot alkaloid Animals Humans Environmental Chemistry Medicine Health risk Chromatography High Pressure Liquid Triticum 030304 developmental biology Pharmacology 0303 health sciences Laboratory methods Chromatography business.industry Secale Degree of confidence business Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 104:546-554 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoacint/qsaa173 |
| Popis: | Background Ergot alkaloids are mycotoxins produced by the fungus Claviceps, which can contaminate grains and pose a health risk to humans and animals. Validation of an ergot alkaloid method in collaborative projects can be challenging due to instability of analytes, a lack of reliable reference materials, and a fully validated reference method. Objective To extensively evaluate performance of a quantitative UHPLC–MS/MS method to detect ten ergot alkaloids at concentrations between 16 and 500 ng/g in grains. Method The method performance was evaluated in the Blinded Method Test (BMT) exercise, which allowed organizers to successfully address the challenges. Forty completely blinded test samples were prepared in an independent laboratory and shipped to a participating laboratory to analyze on two separate days. Results Precision, accuracy, and HorRatr values met or exceeded the U.S. Food and Drug Administration recommendations. The design of the BMT exercise provided a high degree of confidence in data and conclusions drawn. Conclusions The method performed in a manner as expected, and the method can be used by the laboratory for routine testing of wheat and rye grains. Highlights BMT of laboratory methods facilitate validation of tests by evaluating performance in an unbiased manner. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|