Rapid Prototyping: Porous Titanium Alloy Scaffolds Produced by Selective Laser Melting for Bone Tissue Engineering
| Autor: | Sebastian Galonska, Jörg Wiltfang, Eugene Sherry, Stephan T. Becker, Patrick Wollny, Timothy E.L. Douglas, Ingo N.G. Springer, Sureshan Sivananthan, Martin Steiner, Patrick Hans-Heinrich Warnke |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
Biocompatibility Cell Survival Surface Properties Scanning electron microscope Alloy Biomedical Engineering Medicine (miscellaneous) Biocompatible Materials Bioengineering engineering.material Bone tissue Bone and Bones Materials Testing Alloys Fluorescence microscope medicine Humans Selective laser melting Cell Shape Mechanical Phenomena Titanium Osteoblasts Tissue Engineering Tissue Scaffolds Lasers Titanium alloy Osteoblast medicine.anatomical_structure Microscopy Fluorescence engineering Porosity Biomedical engineering |
| Zdroj: | Tissue Engineering Part C: Methods. 15:115-124 |
| ISSN: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2008.0288 |
| Popis: | Selective laser melting (SLM), a method used in the nuclear, space, and racing industries, allows the creation of customized titanium alloy scaffolds with highly defined external shape and internal structure using rapid prototyping as supporting external structures within which bone tissue can grow. Human osteoblasts were cultured on SLM-produced Ti6Al4V mesh scaffolds to demonstrate biocompatibility using scanning electron microscopy (SEM), fluorescence microscopy after cell vitality staining, and common biocompatibility tests (lactate dihydrogenase (LDH), 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), 5-bromo-2-deoxyuridine (BrdU), and water soluble tetrazolium (WST)). Cell occlusion of pores of different widths (0.45-1.2 mm) was evaluated. Scaffolds were tested for resistance to compressive force. SEM investigations showed osteoblasts with well-spread morphology and multiple contact points. Cell vitality staining and biocompatibility tests confirmed osteoblast vitality and proliferation on the scaffolds. Pore overgrowth increased during 6 weeks' culture at pore widths of 0.45 and 0.5 mm, and in the course of 3 weeks for pore widths of 0.55, 0.6, and 0.7 mm. No pore occlusion was observed on pores of width 0.9-1.2 mm. Porosity and maximum compressive load at failure increased and decreased with increasing pore width, respectively. In summary, the scaffolds are biocompatible, and pore width influences pore overgrowth, resistance to compressive force, and porosity. |
| Databáze: | OpenAIRE |
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