Ultrastructural Studies of Spleens, Brains, and Brain Cell Cultures of Mice with Scrapie
| Autor: | E. J. Hinsman, D. P. Gustafson, C. H. Lamar, Margaret Krasovich |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Prions 040301 veterinary sciences Injections Subcutaneous Scrapie Biology Inclusion bodies Injections 0403 veterinary science Mice 03 medical and health sciences medicine Animals Cells Cultured Neurons Mice Inbred ICR Budding Sheep General Veterinary Endoplasmic reticulum Vesicle Brain 04 agricultural and veterinary sciences Microscopy Electron 030104 developmental biology Cell culture Cytoplasm Ultrastructure Female Spleen |
| Zdroj: | Veterinary Pathology. 11:13-19 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/030098587401100102 |
| Popis: | Mouse brains, cell cultures of mouse brains, and spleens from mice with scrapie were examined by electron microscopy. Brains and spleens of 10 scrapie-inoculated and control mice were studied. Seven brain-cell cultures, four of which were from mice inoculated intracerebrally or subcutaneously with scrapie, were examined. Status spongiosus and vacuolated neurons were found in the brains. Structures 35 nm in diameter were seen in the brains of mice inoculated intracerebrally. They were not evident in cell cultures, although a vacuolated structure was found in one such culture. No significant changes were found in the spleens. Through electron microscopy, investigators have sought to show scrapie virus and its associated changes. The established histological diagnostic cri teria - neuronal vacuolation, astrocytic hypertrophy and status spongiosus have been studied also by this means. Natural cases of scrapie have neuronal vacuolation and intravacuolar budding of vesicles and cytoplasmic processes, with some vacuolated neurons containing membrane-bound accumulations of 35-nm particles (2). There are other reports of similar findings without the intravacuolar budding (4), and particles 15-26 X 75 nm were found both in astrocytic processes and nerve cell terminals (20). Electron microscopic examination of experimentally infected animals has included studies of the mouse and rat. Reports on mice indicated areas of sta tus spongiosus, ruptured plasma membranes and curled fragments of mem branes in areas of presynaptic and postsynaptic neuronal terminals, and 35 nm vesicular and tubular structures suggestive of viral particles (10, 17). One report suggested that the small vesicles found within a nerve cell were formed by dilatation of endoplasmic reticulum (13). In rats, neurons were found that contained small inclusion bodies composed of a folded membrane and accu |
| Databáze: | OpenAIRE |
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