Live imaging of mRNA using RNA-stabilized fluorogenic proteins
| Autor: | Samie R. Jaffrey, Jiahui Wu, Hyaeyeong Kim, Sara Zaccara, Marvin E. Tanenbaum, Deepak Khuperkar |
|---|---|
| Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Aptamer
Aptamers Nucleotide/genetics Chemical biology Fluorescence/methods Biochemistry Aptamers Article Fluorescence 03 medical and health sciences RNA Aptamers Live cell imaging Humans Fluorescent Dyes/chemistry Molecular Biology Microscopy Fluorescence/methods 030304 developmental biology Nucleotide/genetics 0303 health sciences Messenger RNA Microscopy Chemistry Bacterial Proteins/genetics RNA Messenger/analysis HEK 293 cells RNA Cell Biology 3. Good health Messenger/analysis HEK293 Cells Molecular Imaging/methods Luminescent Proteins/genetics Biotechnology |
| Zdroj: | Nature methods Nature Methods, 16(9), 862-865. Nature Publishing Group Nature Methods, 16(9), 862. Nature Publishing Group |
| ISSN: | 1548-7091 |
| Popis: | Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA–protein complexes that enable live imaging of mRNA in living cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink