Inactivation of SARS-CoV-2 by Ozonated Glycerol
| Autor: | Hourei Oh, Sachiko Matsuda, Akihiro Kaneko, Yoshimasa Makita, Dulamjav Jamsransuren, Haruko Ogawa, Yohei Takeda |
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| Rok vydání: | 2021 |
| Předmět: |
Glycerol
0301 basic medicine Epidemiology Hand Sanitizers viruses Health Toxicology and Mutagenesis Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology 010501 environmental sciences Antiviral Agents 01 natural sciences Virus Microbiology 03 medical and health sciences chemistry.chemical_compound Ozone Virology Chlorocebus aethiops Severe acute respiratory syndrome coronavirus 2 Animals Hand Hygiene Vero Cells Skin 0105 earth and related environmental sciences Detection limit Original Paper SARS-CoV-2 COVID-19 Viral Load Titer chemistry Ozonated glycerol Antiviral material Fetal bovine serum Food Science |
| Zdroj: | Food and Environmental Virology |
| ISSN: | 1867-0342 1867-0334 |
| DOI: | 10.1007/s12560-021-09485-x |
| Popis: | We evaluated the SARS-CoV-2-inactivation activity of ozonated glycerol (OG). When a viral solution with 1% fetal bovine serum (FBS) was mixed with test solutions at a ratio of 1:19 and incubated for 20 s, OG with ozone concentrations of over 1000 ppm inactivated ≥ 94.38% of the virus. Extension of the reaction time to 1 h led to the inactivation of ≥ 99.82% of the virus (the viral titer was below the detection limit). Extension to 24 h resulted in concentrations over 200 ppm OG inactivating ≥ 99.87% of the virus (the viral titers were below the detection limit). Next, viral solutions with 1, 20, and 40% FBS were mixed with test solutions at a ratio of 1:19 and incubated for 5 min. Whereas the virucidal activity of 500 ppm OG was very limited in the presence of 1% FBS (79.47% inactivation), it increased in the presence of 20 and 40% FBS (95.13 and 97.95% inactivation, respectively; the viral titers were not below the detection limit). Meanwhile, over 1000 ppm OG inactivated ≥ 99.44% of the virus regardless of the FBS concentration (the viral titers were below the detection limit). Extension of the reaction time to 1 h led to 500 ppm OG inactivating ≥ 99.91 and ≥ 99.95% of the virus with 20 and 40% FBS, respectively (the viral titers were below the detection limit). These results suggested that OG might be useful as a virucidal agent against SARS-CoV-2. |
| Databáze: | OpenAIRE |
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