Endothelium‐derived dopamine modulates EFS‐induced contractions of human umbilical vessels

Autor: Valéria Barbosa de Souza, Fabíola Z. Mónica, Guilherme M. Figueiredo Murari, Rafael Campos, José Britto-Júnior, Fernanda Viviane Mariano, André Almeida Schenka, Gilberto De Nucci, Edson Antunes, David Halen Araújo Pinheiro, Alberto Fernando Oliveira Justo
Rok vydání: 2020
Předmět:
Umbilical Veins
Pharmacology
030226 pharmacology & pharmacy
Umbilical Arteries
haloperidol
Norepinephrine
0302 clinical medicine
Short Reports
tyrosine hydroxylase
Tandem Mass Spectrometry
Haloperidol
General Pharmacology
Toxicology and Pharmaceutics
Chemistry
Middle Aged
Receptor antagonist
medicine.anatomical_structure
Neurology
030220 oncology & carcinogenesis
cardiovascular system
Female
human umbilical vein
dopamine
Idazoxan
medicine.drug
Adult
Endothelium
Adolescent
Epinephrine
endothelium
medicine.drug_class
Short Report
RM1-950
EFS
03 medical and health sciences
Young Adult
Dopamine
medicine.artery
Prazosin
medicine
Humans
idazoxan
Adrenergic alpha-Antagonists
prazosin
Tyrosine hydroxylase
L‐NAME
HIBRIDIZAÇÃO
Umbilical artery
Electric Stimulation
Dopamine Antagonists
Endothelium
Vascular
Therapeutics. Pharmacology
Chromatography
Liquid
human umbilical artery
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Pharmacology Research & Perspectives, Vol 8, Iss 4, Pp n/a-n/a (2020)
Pharmacology Research & Perspectives
Popis: Electrical field stimulation (EFS) induces contractions of both snake aorta and human umbilical cord vessels (HUCV) which were dependent on the presence of the endothelium. This study aimed to establish the nature of the mediator(s) responsible for EFS‐induced contractions in HUCV. Rings with or without endothelium from human umbilical artery (HUA) or vein (HUV) were mounted in organ bath chambers containing oxygenated, heated Krebs‐Henseleit's solution. Basal release of dopamine (DA), noradrenaline, and adrenaline was measured by LC‐MS‐MS. Cumulative concentration‐response curves were performed with dopamine in the absence and in the presence of L‐NAME or of dopamine antagonists. EFS studies were performed in the presence and absence of L‐NAME, the α‐adrenergic blockers prazosin and idazoxan, and the dopamine antagonists SCH‐23390 and haloperidol. Tyrosine hydroxylase (TH) and dopa‐decarboxylase (DDC) were studied by immunohistochemistry and fluorescence in situ hybridizations. Basal release of dopamine requires an intact endothelium in both HUA and HUV. TH and DDC are present only in the endothelium of both HUA and HUV as determined by immunohistochemistry. Dopamine induced contractions in HUA only in the presence of L‐NAME. Dopamine‐induced contractions in HUV were strongly potentiated by L‐NAME. The EFS‐induced contractions in both HUA and HUV were potentiated by L‐NAME and inhibited by the D2‐like receptor antagonist haloperidol. The α‐adrenergic antagonists prazosin and idazoxan and the D1‐like receptor antagonist SCH‐23390 had no effect on the EFS‐induced contractions of HUA and HUV. Endothelium‐derived dopamine is a major modulator of HUCV reactivity in vitro.
Databáze: OpenAIRE
Externí odkaz: