Cytotoxic effects of platinum nanoparticles obtained from pomegranate extract by the green synthesis method on the MCF-7 cell line
| Autor: | Hülya Gündüz, Birgütay Şahin, Suleyman Akocak, Ayşenur Aygün, Enes Demir, Fatih Şen, Kubilay Şahin |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Cell Survival
Sonication Infrared spectroscopy Metal Nanoparticles 02 engineering and technology DNA Fragmentation 010402 general chemistry Platinum nanoparticles Biosynthesis 01 natural sciences Monodisperse chemistry.chemical_compound Inhibitory Concentration 50 Colloid and Surface Chemistry Humans Viability assay Propidium iodide Physical and Theoretical Chemistry Fourier transform infrared spectroscopy Platinum Lythraceae Toxicity Cytotoxins Plant Extracts technology industry and agriculture Green Chemistry Technology Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Antineoplastic Agents Phytogenic 0104 chemical sciences MCF-7 chemistry Transmission electron microscopy MCF-7 Cells Ultrasonication Comet Assay 0210 nano-technology Biotechnology Nuclear chemistry |
| Popis: | The study utilizes monodisperse platinum nanoparticles (Pt NPs) biosynthesized from Punica granatum crusts as anti-tumor agents on the human breast cancer cell line, MCF-7. The obtained Pt NPs were fully characterized using the UV–vis spectrum (UV–vis), transmission electron microscopy (TEM), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and Fourier transformation infrared spectroscopy (FTIR). Effectiveness of the Pt NPs was determined by cell viability, propidium iodide staining test, flow cytometry and comet tests on the MCF-7 cancer cell line. Cell survival percentage was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The biosynthesized monodisperse platinum nanoparticles inhibited MCF-7 proliferation with an IC50 of 17.84 µg/ml after 48 h of incubation. Propidium iodide staining demonstrated that the monodisperse Pt NPs induced apoptosis by means of molecular DNA fragmentation. © 2017 Elsevier B.V. 2014-05 The authors would like to thank to Dumlupinar University for funding ( 2014-05 ). Appendix A |
| Databáze: | OpenAIRE |
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