TNF-α modulates angiopoietin-1 expression in rheumatoid synovial fibroblasts via the NF-κB signalling pathway
| Autor: | Aidan G. Gilmartin, James D. Winkler, Michael J Hansbury, A. Colombo, Jeffrey R. Jackson, Paola Zaratin, Boyd B Scott, C. Belpasso |
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| Rok vydání: | 2005 |
| Předmět: |
p38 mitogen-activated protein kinases
Biophysics IκB kinase Biochemistry Arthritis Rheumatoid chemistry.chemical_compound Gene expression Angiopoietin-1 Humans Molecular Biology Cells Cultured Dose-Response Relationship Drug biology Tumor Necrosis Factor-alpha Synovial Membrane NF-kappa B NF-κB Cell Biology Fibroblasts Angiopoietin receptor Cell biology Gene Expression Regulation chemistry Mitogen-activated protein kinase biology.protein Cancer research Signal transduction Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 328:409-414 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2004.12.180 |
| Popis: | Angiopoietin-1 (Ang-1) is one of a family of ligands for the Tie-2 receptor which has been demonstrated to be involved in angiogenesis. Little is known about the regulation of Ang-1 gene expression. We have previously demonstrated that TNF-alpha is able to up-regulate the expression of Ang-1 mRNA in synovial fibroblasts. This present study investigated the signal transduction pathways involved in the TNF-alpha induced expression of Ang-1. TNF-alpha signals primarily through the p38, JNK, MAP kinase, and IKK pathways resulting in the activation of the transcription factors AP-1 and NF-kappa B. Experiments with inhibitors and siRNA for these various signal transduction pathways revealed that TNF-alpha stimulation of Ang-1 expression occurs via the NF-kappa B signal transduction pathway. |
| Databáze: | OpenAIRE |
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