Binding Properties of RNA Quadruplex of SARS-CoV-2 to Berberine Compared to Telomeric DNA Quadruplex

Autor:	Rosario Oliva, Sanjib Mukherjee, Magiliny Manisegaran, Marco Campanile, Pompea Del Vecchio, Luigi Petraccone, Roland Winter
Přispěvatelé:	Oliva, Rosario, Mukherjee, Sanjib, Manisegaran, Magiliny, Campanile, Marco, Del Vecchio, Pompea, Petraccone, Luigi, Winter, Roland
Rok vydání:	2022
Předmět:	Berberine SARS-CoV-2 ligand binding Organic Chemistry COVID-19 hTel G-quadruplex DNA General Medicine Catalysis COVID-19 Drug Treatment Computer Science Applications RNA G-quadruplex Inorganic Chemistry high pressure Humans RNA heterocyclic compounds berberine Physical and Theoretical Chemistry Molecular Biology Spectroscopy Human
Zdroj:	Int J Mol Sci International Journal of Molecular Sciences; Volume 23; Issue 10; Pages: 5690
ISSN:	1000-9035
DOI:	10.3390/ijms23105690
Popis:	Previous studies suggest that berberine, an isoquinoline alkaloid, has antiviral potential and is a possible therapeutic candidate against SARS-CoV-2. The molecular underpinnings of its action are still unknown. Potential targets include quadruplexes (G4Q) in the viral genome as they play a key role in modulating the biological activity of viruses. While several DNA-G4Q structures and their binding properties have been elucidated, RNA-G4Qs such as RG-1 of the N-gene of SARS-CoV-2 are less explored. Using biophysical techniques, the berberine binding thermodynamics and the associated conformational and hydration changes of RG-1 could be characterized and compared with human telomeric DNA-G4Q 22AG. Berberine can interact with both quadruplexes. Substantial changes were observed in the interaction of berberine with 22AG and RG-1, which adopt different topologies that can also change upon ligand binding. The strength of interaction and the thermodynamic signatures were found to dependent not only on the initial conformation of the quadruplex, but also on the type of salt present in solution. Since berberine has shown promise as a G-quadruplex stabilizer that can modulate viral gene expression, this study may also contribute to the development of optimized ligands that can discriminate between binding to DNA and RNA G-quadruplexes.
Databáze:	OpenAIRE
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