The Mouse Gut Microbial Biobank expands the coverage of cultured bacteria
| Autor: | Cheng-Ying Jiang, Yu-Guang Zhou, Linhuan Wu, Jun Wang, Kai Wang, Shuang-Jiang Liu, Yu-Tong Sun, Bing-Bing Bai, Haiying Yu, Dan-Hua Li, Meng-Xuan Du, Hua Xiang, Chang Liu, Yu-Hua Xin, Nan Zhou, Yu-Jing Wang, Yuqin Song, Hongwei Liu, Juncai Ma, Jie Feng |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine China Databases Factual Science Microorganism 030106 microbiology General Physics and Astronomy Genome Article General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences Animals lcsh:Science Symbiosis Cecum Gene Phylogeny Genetics Multidisciplinary Bacteria biology Host (biology) General Chemistry biology.organism_classification Biobank Sequence identity Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology Metagenomics lcsh:Q Microbiome Genome Bacterial |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2041-1723 |
| Popis: | Mice are widely used as experimental models for gut microbiome (GM) studies, yet the majority of mouse GM members remain uncharacterized. Here, we report the construction of a mouse gut microbial biobank (mGMB) that contains 126 species, represented by 244 strains that have been deposited in the China General Microorganism Culture Collection. We sequence and phenotypically characterize 77 potential new species and propose their nomenclatures. The mGMB includes 22 and 17 species that are significantly enriched in ob/ob and wild-type C57BL/6J mouse cecal samples, respectively. The genomes of the 126 species in the mGMB cover 52% of the metagenomic nonredundant gene catalog (sequence identity ≥ 60%) and represent 93–95% of the KEGG-Orthology-annotated functions of the sampled mouse GMs. The microbial and genome data assembled in the mGMB enlarges the taxonomic characterization of mouse GMs and represents a useful resource for studies of host-microbe interactions and of GM functions associated with host health and diseases. Here, the authors established and characterized the mouse gut microbial biobank (mGMB), which includes 244 strains and 126 species that enlarges previous mouse intestinal bacterial collections and represents a resource for studies using mouse models to investigate microbiome-associated health and disease. |
| Databáze: | OpenAIRE |
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