Chlamydia trachomatis induces an upregulation of molecular biomarkers podoplanin, Wilms' tumour gene 1, osteopontin and inflammatory cytokines in human mesothelial cells
| Autor: | De Filippis A, Buommino E, Di Domenico M, Feola A, Brunetti Pierri R, Rizzo A., Brunetti Pierri, Raffaella, DE FILIPPIS, Anna |
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| Přispěvatelé: | De Filippis, A., Buommino, E., di Domenico, M., Feola, A., Brunetti-Pierri, R., Rizzo, A., De Filippis, A, Buommino, E, Di Domenico, M, Feola, A, Brunetti Pierri, R, Brunetti Pierri, Raffaella, DE FILIPPIS, Anna |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mesothelioma 030106 microbiology Inflammation Cell Cycle Proteins Chlamydia trachomatis Biology medicine.disease_cause HeLa Cell Microbiology Epithelium Proinflammatory cytokine Chlamydia Infection 03 medical and health sciences chemistry.chemical_compound Chlamydia trachomati Cell Line Tumor medicine Humans Neoplasm Invasiveness Osteopontin Wilms’ tumour gene 1 Cytokine Nuclear Protein Cell Proliferation Neoplasm Invasivene Epithelial Cell Membrane Glycoproteins Podoplanin Cancer Nuclear Proteins Epithelial Cells Chlamydia Infections medicine.disease Vascular endothelial growth factor chemistry Immunology biology.protein Cytokines Membrane Glycoprotein RNA Splicing Factors medicine.symptom Carcinogenesis Human HeLa Cells |
| Zdroj: | Microbiology (Reading, England). 163(5) |
| ISSN: | 1465-2080 |
| Popis: | Chlamydia trachomatis is the most prevalent infection of the genital tract in women worldwide. C. trachomatis has a tendency to cause persistent infection and induce a state of chronic inflammation, which has been reported to play a role in carcinogenesis. We report that persistent C. trachomatis infection increases the expression of inflammatory tumour cytokines and upregulates molecular biomarkers such as podoplanin, Wilms' tumour gene 1 and osteopontin in primary cultures of mesothelial cells (Mes1) and human mesothelioma cells (NCI). Infection experiments showed that Mes1 and NCI supported the growth of C. trachomatisin vitro, and at an m.o.i. of 4, the inclusion-forming units/cell showed many intracellular inclusion bodies after 3 days of infection. However, after 7 days of incubation, increased proliferative and invasive activity was also observed in Mes1 cells, which was more evident after 14 days of incubation. ELISA analysis revealed an increase in vascular endothelial growth factor, IL-6, IL-8, and TNF-α release in Mes1 cells infected for a longer period (14 days). Finally, real-time PCR analysis revealed a strong induction of podoplanin, Wilms' tumour gene 1 and osteopontin gene expression in infected Mes1 cells. The aim of the present study was to investigate the inflammatory response elicited by C. trachomatis persistent infection and the role played by inflammation in cell proliferation, secretion of proinflammatory cytokines and molecular biomarkers of cancer. The results of this study suggest that increased molecular biomarkers of cancer by persistent inflammation from C. trachomatis infection might support cellular transformation, thus increasing the risk of cancer. |
| Databáze: | OpenAIRE |
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