Impact of Nonspecific Adsorption to Metal Surfaces in Ion Pair-RP LC-MS Impurity Analysis of Oligonucleotides
| Autor: | Michael Donegan, Martin Gilar, Guilherme J Guimaraes, J. Michael Sutton, Michael G. Bartlett |
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| Rok vydání: | 2021 |
| Předmět: |
Analyte
Chemistry Clinical Biochemistry Inorganic chemistry Oligonucleotides Pharmaceutical Science Chemical modification Reproducibility of Results Analytical Chemistry Metal Adsorption Liquid chromatography–mass spectrometry Impurity Tandem Mass Spectrometry visual_art Phase (matter) Drug Discovery visual_art.visual_art_medium Fluidics Spectroscopy Chromatography Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 208 |
| ISSN: | 1873-264X |
| Popis: | Nonspecific adsorption has been a consistent challenge in the analysis of oligonucleotides. Nonspecific adsorption is a result of interactions between charged acidic analytes and adsorption sites present in metallic surfaces located in the fluidic path of chromatography systems. Due to their high surface area, adsorption to column frits is especially concerning. Poor peak shape, low recovery and compromised LOQ have been associated with this phenomenon. Alternative methods including substitution of stainless steel for different hardware materials and mobile phase additives have been explored in an attempt to minimize this issue. Chemical modification of metal surfaces using hybrid surface technology (HST) by-passes the limitation of stainless steel construction material by forming a hybrid organic/inorganic layer that acts as a barrier and limits nonspecific interactions. In this study we explore the implications of this new technology in sensitive analysis and determination of relative impurity levels of oligonucleotides. Higher relative impurity levels and better reproducibility were obtained with columns using HST. |
| Databáze: | OpenAIRE |
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