A method that allows easy characterization of tumor-infiltrating lymphocytes
| Autor: | Hildegund C.J. Ertl, Anthony P Wlazlo, Magdalena Blaszczyk-Thurin, Zhiquan Xiang, Wynetta Giles-Davis, Dariusz W Kowalczyk |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pathology
medicine.medical_specialty T cell Papillomavirus E7 Proteins Immunology Population Biology CD8-Positive T-Lymphocytes Lymphocyte Activation Cancer Vaccines Lymphocytic Infiltrate Mice Lymphocytes Tumor-Infiltrating Antigen medicine Tumor Cells Cultured Immunology and Allergy Animals education Antigens Viral Tumor Matrigel education.field_of_study Mice Inbred BALB C Tumor-infiltrating lymphocytes Papillomavirus Infections Viral Vaccines Neoplasms Experimental Oncogene Proteins Viral Cytotoxicity Tests Immunologic Cellular Infiltrate Mice Inbred C57BL Drug Combinations Tumor Virus Infections medicine.anatomical_structure Cancer research Female Proteoglycans Collagen Laminin Chemokines CD8 |
| Zdroj: | Journal of immunological methods. 253(1-2) |
| ISSN: | 0022-1759 |
| Popis: | A method was developed to compare the lymphocytic infiltrates in regressing vs. progressing experimental mouse tumors using a model for human papillomavirus-16 (HPV-16) oncoprotein-linked cancer. Tumor cells mixed with matrigel, composed of natural matrix substances that provide a basement membrane structure for adherent cells, were inoculated into mice vaccinated with an efficacious vaccine to the E7 oncoprotein or a vaccine to a control antigen. The tumor cells remained within the solidified gel and recruited a cellular infiltrate that could readily be analyzed upon removal of the gelatinous mass containing progressing or regressing tumors. The results show that tumors recruit activated CD8 + T cells regardless of their antigen specificity. In regressing tumors expressing an appropriate target antigen for the vaccine-induced CD8 + T cells, a strong increase of the tumor antigen-specific T cell population was observed over time. Progressing tumors that lacked the target antigen for the activated CD8 + T cell population did not show this selective enrichment. |
| Databáze: | OpenAIRE |
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