Picroscope: low-cost system for simultaneous longitudinal biological imaging
| Autor: | Gary L. Mantalas, Kateryna Voitiuk, Erik Jung, Jayden M. Ross, Pattawong Pansodtee, Mircea Teodorescu, Pierre V. Baudin, Marco Rolandi, Yohei Rosen, Helen Rankin Willsey, Mohammed A. Mostajo-Radji, Alex A. Pollen, John Selberg, Tomasz J. Nowakowski, Victoria T. Ly, Sergio A. Cordero, David Haussler, Sofie R. Salama, Spencer T. Seiler |
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| Rok vydání: | 2021 |
| Předmět: |
Computer science
QH301-705.5 Xenopus Medicine (miscellaneous) Bioengineering General Biochemistry Genetics and Molecular Biology Article Imaging Mammalian tissue Imaging Three-Dimensional Developmental biology Animals Computer vision Biology (General) Zebrafish Mammals Behavior biology Behavior Animal business.industry Animal Biological techniques fungi Planarians Longitudinal imaging biology.organism_classification Planaria Organoids Three-Dimensional Zebrafish embryo Biomedical Imaging Artificial intelligence Generic health relevance General Agricultural and Biological Sciences Biological imaging business Biotechnology |
| Zdroj: | Communications biology, vol 4, iss 1 Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021) Communications Biology |
| Popis: | Simultaneous longitudinal imaging across multiple conditions and replicates has been crucial for scientific studies aiming to understand biological processes and disease. Yet, imaging systems capable of accomplishing these tasks are economically unattainable for most academic and teaching laboratories around the world. Here, we propose the Picroscope, which is the first low-cost system for simultaneous longitudinal biological imaging made primarily using off-the-shelf and 3D-printed materials. The Picroscope is compatible with standard 24-well cell culture plates and captures 3D z-stack image data. The Picroscope can be controlled remotely, allowing for automatic imaging with minimal intervention from the investigator. Here, we use this system in a range of applications. We gathered longitudinal whole organism image data for frogs, zebrafish, and planaria worms. We also gathered image data inside an incubator to observe 2D monolayers and 3D mammalian tissue culture models. Using this tool, we can measure the behavior of entire organisms or individual cells over long-time periods. Ly et al. report a multi-well imaging system (the Picroscope) that can perform longitudinal bright-field z-stack imaging for 24 wells simultaneously. They demonstrate its capability by imaging zebrafish embryos, planaria worms, development of Xenopus tropicalis over a 28-h period and embryonic stem cell monolayer and organoids inside an incubator. |
| Databáze: | OpenAIRE |
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