High dietary sodium causes dyssynchrony of the renal molecular clock in rats
| Autor: | Martin E. Young, Kaehler J. Roth, Jennifer S. Pollock, Jonathan Brett Heimlich, Joshua S. Speed, Malgorzata Kasztan, David M. Pollock, Brandon M. Fox, Jermaine G. Johnston, Karen L. Gamble, Bryan K. Becker, Kelly A. Hyndman, Chunhua Jin |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Physiology Sodium CLOCK Proteins chemistry.chemical_element 030204 cardiovascular system & hematology Biology Kidney 03 medical and health sciences 0302 clinical medicine Dietary Sodium Internal medicine Cell autonomous medicine Animals Circadian rhythm Sodium Chloride Dietary Molecular clock Endothelins Sodium Dietary Feeding Behavior Period Circadian Proteins Circadian Rhythm Rats 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Research Article |
| Zdroj: | American Journal of Physiology-Renal Physiology. 314:F89-F98 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.00028.2017 |
| Popis: | Speed JS, Hyndman KA, Roth K, Heimlich JB, Kasztan M, Fox BM, Johnston JG, Becker BK, Jin C, Gamble KL, Young ME, Pollock JS, Pollock DM. High dietary sodium causes dyssynchrony of the renal molecular clock in rats. Am J Physiol Renal Physiol 314: F89–F98, 2018. First published September 27, 2017; doi:10.1152/ajprenal.00028.2017.—Dyssynchrony of circadian rhythms is associated with various disorders, including cardiovascular and metabolic diseases. The cell autonomous molecular clock maintains circadian control; however, environmental factors that may cause circadian dyssynchrony either within or between organ systems are poorly understood. Our laboratory recently reported that the endothelin (ET-1) B (ETB) receptor functions to facilitate Na+ excretion in a time of day-dependent manner. Therefore, the present study was designed to determine whether high salt (HS) intake leads to circadian dyssynchrony within the kidney and whether the renal endothelin system contributes to control of the renal molecular clock. We observed that HS feeding led to region-specific alterations in circadian clock components within the kidney. For instance, HS caused a significant 5.5-h phase delay in the peak expression of Bmal1 and suppressed Cry1 and Per2 expression in the renal inner medulla, but not the renal cortex, of control rats. The phase delay in Bmal1 expression appears to be mediated by ET-1 because this phenomenon was not observed in the ETB-deficient rat. In cultured inner medullary collecting duct cells, ET-1 suppressed Bmal1 mRNA expression. Furthermore, Bmal1 knockdown in these cells reduced epithelial Na+ channel expression. These data reveal that HS feeding leads to intrarenal circadian dyssynchrony mediated, in part, through activation of ETB receptors within the renal inner medulla. |
| Databáze: | OpenAIRE |
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