One-step integration of multiple genes into the oleaginous yeast Yarrowia lipolytica
| Autor: | Sheng Yang, Yu Jiang, Li Zhu, Linna Han, Mei Ge, Daijie Chen, Shuliang Gao |
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| Rok vydání: | 2014 |
| Předmět: |
Genetics
Microbial Time Factors biology Fungal genetics Yarrowia Bioengineering Locus (genetics) General Medicine biology.organism_classification beta Carotene Applied Microbiology and Biotechnology DNA Ribosomal Yeast Biosynthetic Pathways Metabolic engineering Biochemistry Metabolic Engineering Homologous recombination DNA Fungal Homologous Recombination Functional genomics Gene Molecular Biology Biotechnology |
| Zdroj: | Biotechnology letters. 36(12) |
| ISSN: | 1573-6776 |
| Popis: | Yarrowia lipolytica is an unconventional yeast, and is generally recognized as safe (GRAS). It provides a versatile fermentation platform that is used commercially to produce many added-value products. Here we report a multiple fragment assembly method that allows one-step integration of an entire β-carotene biosynthesis pathway (~11 kb, consisting of four genes) via in vivo homologous recombination into the rDNA locus of the Y. lipolytica chromosome. The highest efficiency was 21 %, and the highest production of β-carotene was 2.2 ± 0.3 mg per g dry cell weight. The total procedure was completed in less than one week, as compared to a previously reported sequential gene integration method that required n weeks for n genes. This time-saving method will facilitate synthetic biology, metabolic engineering and functional genomics studies of Y. lipolytica. |
| Databáze: | OpenAIRE |
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