Amelioration of pulmonary aflatoxicosis by green tea extract: An in vivo study
| Autor: | Maha Anani, Nassar Ayoub Abdellatif, Shimaa Antar Fareed, Dalia Abdallah El-Shafei, Eman Ahmed Alaa El-Din, Amal Nooredeen Ahmed Allithy, Heba El-Sayed Mostafa |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Aflatoxin B1 Antioxidant medicine.medical_treatment Anti-Inflammatory Agents H&E stain Green tea extract Pharmacology Toxicology medicine.disease_cause Antioxidants Camellia sinensis Proinflammatory cytokine chemistry.chemical_compound Malondialdehyde medicine Animals Lung Inflammation Tea biology Plant Extracts business.industry Mycotoxicosis Glutathione Rats Oxidative Stress medicine.anatomical_structure Liver chemistry Myeloperoxidase biology.protein Cytokines business Biomarkers Oxidative stress |
| Zdroj: | Toxicon. 189:48-55 |
| ISSN: | 0041-0101 |
| Popis: | Aflatoxins (AFB1) are mycotoxins known to be associated with human and animal diseases. The lung is a at risk from AFB1exposure either via inhalation or circulation. Green tea consumption is increasing over time due to widespread popularity as antioxidants, anti-inflammatory, and cytoprotective agents. Therefore, we attempted to study the lung toxicity caused by AFB1 and the possible ameliorating effect of green tea extract. Forty adult male albino rats were divided into five groups; Group I: Untreated control group, Group II (vehicle): Each rat received 1 ml of olive oil, Group III (GTE): Each rat received Camellia sinensis, green tea extract (30 mg/kg/day), Group IV(AFB1): Each rat received (50 μg/kg/day of AFB1). Group V (AFB1+ GTE): Each rat received the same previously mentioned doses of AFB1 in addition to GTE concomitantly. All treatments were orally gavaged for 8 weeks then rats were sacrificed. Serum levels of pro-inflammatory (IL-1β, TNF-α, IL-6) and anti-inflammatory (IL-10) cytokines were measured, lung tissues’ oxidative stress indices were also measured in addition to the histopathological study which was performed by using hematoxylin & eosin and Masson trichrome stains. Morphometric and statistical analyses were also performed. Oral gavage of AFB1 resulted in significant histopathological changes in the lung tissues, in the form of variable degrees of congestion, hemorrhage, interstitial inflammation with infiltration by chronic inflammatory cells, interstitial fibrosis, bronchitis, vasculitis and fibrous thickening of arterial walls. Inflammation was evident by elevated levels of pro-inflammatory cytokines and a declined level of anti-inflammatory cytokines. Also, oxidative stress was evident by increased levels of Malondialdehyde (MDA), Myeloperoxidase (MPO), and decreased levels of total glutathione (tGSH) and Catalase (CAT). The histopathological changes, inflammatory cytokines, and oxidative stress markers were significantly decreased during concomitant administration of green tea extract in (AFB1+ GTE) group. Aflatoxin B1 has deleterious effects on the lung tissue that could be minimized by concomitant administration of Green tea extract owing to its anti-inflammatory, antioxidant, and protective properties. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect