Neutrophils are indispensable for hematopoietic stem cell mobilization induced by interleukin-8 in mice

Autor: Ronald van Os, Marianke L J Van Schie, Sofie Starckx, Ivan J. D. Lindley, Annunciata Vecchi, Alberto Mantovani, Evert Jan F M De Kruijf, Ghislain Opdenakker, Willem E. Fibbe, Perry Verzaal, Roel Willemze, J.F.M. Pruijt
Přispěvatelé: Damage and Repair in Cancer Development and Cancer Treatment (DARE), Stem Cell Aging Leukemia and Lymphoma (SALL)
Jazyk: angličtina
Rok vydání: 2002
Předmět:
EXPRESSION
Neutropenia
Time Factors
bone marrow
Neutrophils
Population
Biology
G-CSF
PERIPHERAL-BLOOD
GELATINASE-B
BONE-MARROW CELLS
metalloproteinases
RADIOPROTECTIVE CAPACITY
Mice
medicine
Animals
adhesion molecules
Interleukin 8
Progenitor cell
education
Hematopoietic Stem Cell Mobilization
Cells
Cultured
education.field_of_study
Mice
Inbred BALB C
Multidisciplinary
Dose-Response Relationship
Drug
RAPID MOBILIZATION
Interleukin-8
Antibodies
Monoclonal
ADHESION MOLECULE-1
hemic and immune systems
Biological Sciences
COLONY-STIMULATING FACTOR
medicine.disease
Colony-stimulating factor
Flow Cytometry
Hematopoietic Stem Cells
Neutrophilia
Recombinant Proteins
medicine.anatomical_structure
Matrix Metalloproteinase 9
PROGENITOR CELLS
Immunology
Bone marrow
medicine.symptom
MMP-9
RHESUS-MONKEYS
Zdroj: Proceedings of the National Academy of Science of the United States of America, 99(9), 6228-6233. NATL ACAD SCIENCES
ISSN: 0027-8424
DOI: 10.1073/pnas.092112999
Popis: The CXC chemokine interleukin-8 (IL-8/CXCL8) induces rapid mobilization of hematopoietic progenitor cells (HPCs). Previously we showed that mobilization could be prevented completely in mice by pretreatment with neutralizing antibodies against the β2-integrin LFA-1 (CD11a). In addition, murine HPCs do not express LFA-1, indicating that mobilization requires a population of accessory cells. Here we show that polymorphonuclear cells (PMNs) serve as key regulators in IL-8-induced HPC mobilization. The role of PMNs was studied in mice rendered neutropenic by administration of a single injection of antineutrophil antibodies. Absolute neutropenia was observed up to 3–5 days with a rebound neutrophilia at day 7. The IL-8-induced mobilizing capacity was reduced significantly during the neutropenic phase, reappeared with recurrence of the PMNs, and was increased proportionally during the neutrophilic phase. In neutropenic mice, the IL-8-induced mobilizing capacity was restored by the infusion of purified PMNs but not by infusion of mononuclear cells. Circulating metalloproteinase gelatinase B (MMP-9) levels were detectable only in neutropenic animals treated with PMNs in combination with IL-8, showing thatin vivoactivated PMNs are required for the restoration of mobilization. However, IL-8-induced mobilization was not affected in MMP-9-deficient mice, indicating that MMP-9 is not indispensable for mobilization. These data demonstrate that IL-8-induced mobilization of HPCs requires thein vivoactivation of circulating PMNs.
Databáze: OpenAIRE
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