Flavocoxid attenuates airway inflammation in ovalbumin-induced mouse asthma model
| Autor: | Ghada M. Suddеk, Rania R. Abdеlaziz, Mohammеd kh Еlmahdy |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Flavocoxid Ovalbumin Inflammation Toxicology medicine.disease_cause Catechin 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine medicine Animals Lung Asthma medicine.diagnostic_test biology business.industry General Medicine respiratory system medicine.disease Malondialdehyde respiratory tract diseases Disease Models Animal Drug Combinations Oxidative Stress 030104 developmental biology Bronchoalveolar lavage chemistry 030220 oncology & carcinogenesis Interleukin 13 Immunology biology.protein medicine.symptom business Oxidative stress Biomarkers |
| Zdroj: | Chemico-biological interactions. 292 |
| ISSN: | 1872-7786 |
| Popis: | Asthma is a common airways inflammatory disease. This study provides evidence on the efficacy of flavocoxid against ovalbumin (OVA)-induced allergic airways inflammation in a mouse model of asthma. Airway inflammation was induced by intrapеritonеal injection of 10 mg ovalbumin (OVA) on day zero and day 7 followed by OVA challenge starting from 14th day to 16th day. Beclomethasone; a standard anti-inflammatory agent was selected as a drug in asthma. Flavocoxid (20 mg/kg, i. p.) was administered on day zero till 16th day followed by OVA challenge. At the end of the study, lung weight index, bronchoalveolar lavage fluid (BALF) content of total and differential WBCs, interleukin-13(IL-13), in addition to lung tissue nitrate/nitrite (NO) and oxidative stress biomarkers were measured. Also, histological and immunohistochemical analysis were conducted. Daily i. p. injection of flavocoxid (20 mg/kg) significantly improved airway inflammation. Inflammatory cells in BALF, malondialdehyde (MDA), NO and IL-13 significantly declined with concomitant increase in superoxide dismutase (SOD) activity. Histopathological examination and immunohistochеmical staining of mast cells were correlated with observed biochemical improvements. Collectively, these results demonstrate that flavocoxid mitigates the allergic airway inflammation induced by ovalbumin through attenuation of IL-13, NO expressions and oxidative stress. |
| Databáze: | OpenAIRE |
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